Identification of a Novel Imprinted Transcript in the Porcine
Animals
Base Sequence
/ genetics
DNA Methylation
/ genetics
Epigenesis, Genetic
/ genetics
Epigenome
/ genetics
Exons
/ genetics
Fetus
GTP-Binding Protein alpha Subunits, Gs
/ genetics
Genome
/ genetics
Genomic Imprinting
/ genetics
Parthenogenesis
Promoter Regions, Genetic
/ genetics
Sequence Analysis, RNA
/ methods
Swine
/ genetics
Transcriptome
/ genetics
GNAS
RNA-sequencing
differentially methylated region
fetus
genomic imprinting
parthenogenetic
parthenote
porcine
whole genome bisulfite sequencing
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
14 01 2020
14 01 2020
Historique:
received:
09
12
2019
revised:
10
01
2020
accepted:
13
01
2020
entrez:
18
1
2020
pubmed:
18
1
2020
medline:
30
9
2020
Statut:
epublish
Résumé
Genomic imprinting in domestic animals contributes to the variance of performance traits. However, research remains to be done on large-scale detection of epigenetic landscape of porcine imprinted loci including the GNAS complex locus. The purpose of this study was to generate porcine parthenogenetic fetuses and comprehensively identify imprinting patterns of the GNAS locus in transcript levels. To this end, both normally fertilized and bimaternal (uniparental) parthenogenetic porcine fetuses were generated, and whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) were performed to construct methylome and transcriptome, respectively. Differentially methylated regions (DMRs) between the fetuses were identified through methylome analysis, and parental-origin-specific expression patterns of transcripts were examined with transcriptome. As a result, three major DMRs were identified: paternally methylated
Identifiants
pubmed: 31947640
pii: genes11010096
doi: 10.3390/genes11010096
pmc: PMC7017182
pii:
doi:
Substances chimiques
GTP-Binding Protein alpha Subunits, Gs
EC 3.6.5.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Genome Res. 2016 Feb;26(2):256-62
pubmed: 26631489
Curr Biol. 2008 Nov 25;18(22):1735-41
pubmed: 19026546
Nat Biotechnol. 2010 Oct;28(10):1057-68
pubmed: 20944598
Reproduction. 2008 Jan;135(1):29-40
pubmed: 18159081
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8715-20
pubmed: 9671744
Nat Genet. 2006 Mar;38(3):350-5
pubmed: 16462745
Cell Metab. 2009 Jun;9(6):548-55
pubmed: 19490909
Mol Biol Rep. 2015 Feb;42(2):345-53
pubmed: 25270250
J Endocrinol Invest. 2017 Apr;40(4):347-356
pubmed: 27995443
Nat Genet. 2015 May;47(5):544-9
pubmed: 25848752
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15475-80
pubmed: 9860993
Animal. 2010 May;4(5):672-81
pubmed: 22444119
Annu Rev Anim Biosci. 2014 Feb;2:23-40
pubmed: 25384133
Mol Cell Biol. 2000 Aug;20(16):5808-17
pubmed: 10913164
Pharmacol Ther. 2007 Aug;115(2):271-91
pubmed: 17588669
J Clin Endocrinol Metab. 2014 Apr;99(4):E724-8
pubmed: 24438374
J Biol Chem. 2006 Jul 14;281(28):18989-99
pubmed: 16672216
Am J Hum Genet. 2005 May;76(5):804-14
pubmed: 15800843
Nat Genet. 2004 Aug;36(8):894-9
pubmed: 15273687
Nat Genet. 2004 Aug;36(8):818-26
pubmed: 15273686
PLoS One. 2017 Nov 30;12(11):e0188278
pubmed: 29190766
J Hum Genet. 2016 Feb;61(2):87-94
pubmed: 26377239
PLoS Genet. 2011 Mar;7(3):e1001347
pubmed: 21455290
Nat Rev Genet. 2001 Jan;2(1):21-32
pubmed: 11253064
Proc Natl Acad Sci U S A. 1988 Apr;85(7):2081-5
pubmed: 3127824
Genome Res. 2012 Oct;22(10):2008-17
pubmed: 22722343
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
PLoS One. 2011;6(11):e27288
pubmed: 22102886
Methods Mol Biol. 2016;1418:335-51
pubmed: 27008022
Gene Expr Patterns. 2012 Jan-Feb;12(1-2):18-23
pubmed: 22067442
Soc Reprod Fertil Suppl. 2009;66:245-64
pubmed: 19848292
Transgenic Res. 2017 Feb;26(1):153-163
pubmed: 27554374
Proc Natl Acad Sci U S A. 2005 May 17;102(20):7386-91
pubmed: 15883378
Clin Epigenetics. 2015 Nov 14;7:123
pubmed: 26583054