Variation among S-locus haplotypes and among stylar RNases in almond.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 01 2020
17 01 2020
Historique:
received:
22
04
2019
accepted:
31
12
2019
entrez:
19
1
2020
pubmed:
19
1
2020
medline:
20
11
2020
Statut:
epublish
Résumé
In many plant species, self-incompatibility systems limit self-pollination and mating among relatives. This helps maintain genetic diversity in natural populations but imposes constraints in agriculture and plant breeding. In almond [Prunus dulcis (Mill.) D.A. Webb], the specificity of self-incompatibility is mainly determined by stylar ribonuclease (S-RNase) and S-haplotype-specific F-box (SFB) proteins, both encoded within a complex locus, S. Prior to this research, a nearly complete sequence was available for one S-locus haplotype. Here, we report complete sequences for four haplotypes and partial sequences for 11 haplotypes. Haplotypes vary in sequences of genes (particularly S-RNase and SFB), distances between genes and numbers and positions of long terminal repeat transposons. Haplotype variation outside of the S-RNase and SFB genes may help maintain functionally important associations between S-RNase and SFB alleles. Fluorescence-based assays were developed to distinguish among some S-RNase alleles. With three-dimensional modelling of five S-RNase proteins, conserved active sites were identified and variation was observed in electrostatic potential and in the numbers, characteristics and positions of secondary structural elements, loop anchoring points and glycosylation sites. A hypervariable region on the protein surface and differences in the number, location and types of glycosylation sites may contribute to determining S-RNase specificity.
Identifiants
pubmed: 31953457
doi: 10.1038/s41598-020-57498-6
pii: 10.1038/s41598-020-57498-6
pmc: PMC6969032
doi:
Substances chimiques
F-Box Proteins
0
Plant Proteins
0
Ribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
583Subventions
Organisme : Horticulture Australia (Horticulture Australia Limited)
ID : AL12015
Pays : International
Organisme : Department of Education and Training | Australian Research Council (ARC)
ID : DP120100900
Pays : International
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