Lysine acetylation of the housekeeping sigma factor enhances the activity of the RNA polymerase holoenzyme.
Acetylation
Amino Acid Sequence
Bacterial Proteins
/ metabolism
DNA-Directed RNA Polymerases
/ metabolism
Gene Expression Regulation, Bacterial
Genes, Essential
Holoenzymes
/ metabolism
Lysine
/ metabolism
Mutation
/ genetics
Promoter Regions, Genetic
Protein Binding
Sigma Factor
/ chemistry
Streptomyces
/ genetics
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
18 03 2020
18 03 2020
Historique:
accepted:
04
01
2020
revised:
28
12
2019
received:
03
10
2019
pubmed:
24
1
2020
medline:
19
5
2020
entrez:
24
1
2020
Statut:
ppublish
Résumé
Protein lysine acetylation, one of the most abundant post-translational modifications in eukaryotes, occurs in prokaryotes as well. Despite the evidence of lysine acetylation in bacterial RNA polymerases (RNAPs), its function remains unknown. We found that the housekeeping sigma factor (HrdB) was acetylated throughout the growth of an actinobacterium, Streptomyces venezuelae, and the acetylated HrdB was enriched in the RNAP holoenzyme complex. The lysine (K259) located between 1.2 and 2 regions of the sigma factor, was determined to be the acetylated residue of HrdB in vivo by LC-MS/MS analyses. Specifically, the label-free quantitative analysis revealed that the K259 residues of all the HrdB subunits were acetylated in the RNAP holoenzyme. Using mutations that mimic or block acetylation (K259Q and K259R), we found that K259 acetylation enhances the interaction of HrdB with the RNAP core enzyme as well as the binding activity of the RNAP holoenzyme to target promoters in vivo. Taken together, these findings provide a novel insight into an additional layer of modulation of bacterial RNAP activity.
Identifiants
pubmed: 31970401
pii: 5714263
doi: 10.1093/nar/gkaa011
pmc: PMC7049703
doi:
Substances chimiques
Bacterial Proteins
0
Holoenzymes
0
Sigma Factor
0
DNA-Directed RNA Polymerases
EC 2.7.7.6
Lysine
K3Z4F929H6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2401-2411Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Elife. 2015 Sep 08;4:
pubmed: 26349034
Mol Microbiol. 2013 Mar;87(6):1223-36
pubmed: 23347076
Int J Biochem Cell Biol. 2015 Feb;59:193-202
pubmed: 25456444
Mol Microbiol. 2017 Apr;104(2):278-293
pubmed: 28118511
J Bacteriol. 2013 Sep;195(18):4174-86
pubmed: 23852870
J Bacteriol. 2006 Aug;188(15):5460-8
pubmed: 16855235
J Biol Chem. 2012 May 4;287(19):15590-601
pubmed: 22416131
Mol Cell Biol. 2008 Jan;28(1):227-36
pubmed: 17938198
PLoS Pathog. 2016 Mar 04;12(3):e1005458
pubmed: 26943369
Emerg Microbes Infect. 2018 Mar 21;7(1):34
pubmed: 29559631
Microbiol Mol Biol Rev. 2015 Sep;79(3):321-46
pubmed: 26179745
J Biol Chem. 2012 Sep 14;287(38):32147-60
pubmed: 22829598
mBio. 2019 Apr 9;10(2):
pubmed: 30967470
PLoS One. 2010 Dec 31;5(12):e15123
pubmed: 21217812
J Proteome Res. 2013 Feb 1;12(2):844-51
pubmed: 23294111
Nucleic Acids Res. 2019 Jan 25;47(2):621-633
pubmed: 30371884
Adv Microb Physiol. 2002;46:47-110
pubmed: 12073657
Mol Microbiol. 2012 Jul;85(2):326-44
pubmed: 22651816
mBio. 2017 Nov 28;8(6):
pubmed: 29184018
J Proteomics. 2014 Jun 25;106:260-9
pubmed: 24768905
mSystems. 2016 May;1(3):
pubmed: 27376153
PLoS One. 2013 Jun 27;8(6):e67186
pubmed: 23826228
J Biotechnol. 2016 Feb 10;219:57-8
pubmed: 26718561
Genom Data. 2014 Dec;2:110-113
pubmed: 25089258
Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):6653-8
pubmed: 27247389
Proteomics. 2015 Jul;15(13):2152-7
pubmed: 25900529
Mol Syst Biol. 2014 Nov 27;10:762
pubmed: 25518064
J Infect Dis. 2017 Nov 15;216(8):1018-1026
pubmed: 28329249
Nucleic Acids Res. 1997 Jul 1;25(13):2566-73
pubmed: 9185565
J Bacteriol. 1990 Jun;172(6):3367-78
pubmed: 2160942
PLoS One. 2015 Jun 22;10(6):e0131169
pubmed: 26098117
Annu Rev Microbiol. 2019 Sep 8;73:111-132
pubmed: 31091420
Sci Rep. 2016 Aug 03;6:30837
pubmed: 27484197
Mol Microbiol. 2018 Jan;107(1):116-131
pubmed: 29105190
Mol Microbiol. 2012 Jun;84(6):1033-49
pubmed: 22582857
Trends Microbiol. 2017 Sep;25(9):768-779
pubmed: 28462789
Nucleic Acids Res. 2017 May 19;45(9):5349-5358
pubmed: 28398568
Front Microbiol. 2018 May 23;9:941
pubmed: 29875739
J Microbiol Biotechnol. 2008 Sep;18(9):1529-36
pubmed: 18852508
J Proteomics. 2017 Feb 23;155:63-72
pubmed: 28034645
Cell Mol Life Sci. 2004 Aug;61(16):2020-30
pubmed: 15316652
Sci Rep. 2016 Jan 29;6:20108
pubmed: 26822828
J Bacteriol. 2017 Jul 25;199(16):
pubmed: 28439035
Nucleic Acids Res. 2016 Mar 18;44(5):1979-88
pubmed: 26847092
Mol Microbiol. 2011 Sep;81(5):1190-204
pubmed: 21696463
J Proteome Res. 2014 Jul 3;13(7):3294-302
pubmed: 24874924
Mol Cell. 2013 Jul 25;51(2):265-72
pubmed: 23830618
J Proteome Res. 2015 Feb 6;14(2):1275-86
pubmed: 25621733
Mol Microbiol. 2015 Dec;98(5):847-63
pubmed: 26264774
FEBS Lett. 2017 Jul;591(13):1958-1965
pubmed: 28542702
J Mol Biol. 2004 Sep 10;342(2):383-401
pubmed: 15327942
Appl Microbiol Biotechnol. 2015 Feb;99(3):1399-413
pubmed: 25487885
Mol Microbiol. 1992 May;6(9):1133-9
pubmed: 1350315
J Bacteriol. 2014 Dec;196(24):4253-67
pubmed: 25266378
Methods Enzymol. 2003;370:73-82
pubmed: 14712635
Nucleic Acids Res. 2013 Jun;41(11):5679-91
pubmed: 23605043
Mol Microbiol. 2010 Jun 1;76(5):1162-74
pubmed: 20345663
Science. 2010 Feb 19;327(5968):1004-7
pubmed: 20167787
Front Microbiol. 2019 Jul 12;10:1604
pubmed: 31354686
J Biol Chem. 2016 Oct 14;291(42):22315-22326
pubmed: 27566542
Gene. 1997 Sep 1;196(1-2):31-42
pubmed: 9322738
Elife. 2017 Jan 09;6:
pubmed: 28067618
J Biol Chem. 2018 May 11;293(19):7367-7375
pubmed: 29581236
Front Microbiol. 2016 Nov 29;7:1918
pubmed: 27965645
J Proteome Res. 2016 Aug 5;15(8):2567-78
pubmed: 27323652
Biochim Biophys Acta. 2016 Oct;1864(10):1372-401
pubmed: 27296530
PLoS One. 2014 Apr 22;9(4):e94816
pubmed: 24756028
Gene. 1992 Jul 1;116(1):43-9
pubmed: 1628843
Metallomics. 2015 Apr;7(4):702-9
pubmed: 25697558
J Microbiol. 2019 May;57(5):388-395
pubmed: 30721456