Construction of yeast producing patchoulol by global metabolic engineering strategy.
Saccharomyces cerevisiae
global metabolic engineering
mevalonate pathway
patchoulol
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
20
11
2019
revised:
16
01
2020
accepted:
21
01
2020
pubmed:
26
1
2020
medline:
13
7
2021
entrez:
26
1
2020
Statut:
ppublish
Résumé
Patchoulol is a sesquiterpene alcohol found in the leaves of the patchouli plant that can be extracted by steam distillation. Notably, patchoulol is an essential natural product frequently used in the chemical industry. However, patchouli produces an insignificant amount of patchoulol, not to mention steam distillation, and requires a lot of energy and time. Recombinant microorganisms that can be cultured in mild conditions and can produce patchoulol from renewable biomass resources may be a promising alternative. We previously developed the global metabolic engineering strategy (GMES), which produces a comprehensive metabolic modification in yeast, using the cocktail δ-integration method. In this study, we aimed to produce patchoulol by modifying engineered yeast. The expression of nine genes involved in patchoulol synthesis was modulated using GMES. Regarding patchoulol production, the resultant strain, YPH499/PAT167/MVA442, showed a concentration of 42.1 mg/L, a production rate of 8.42 mg/L/d, and a yield of 2.05 mg/g-glucose, respectably. These concentration values, production rate, and yield obtained through batch-fermentation in this study were high level when compared to previously reported recombinant microorganism studies. GMES could be used as a potential strategy for producing secondary metabolites from plants in recombinant Saccharomyces cerevisiae.
Substances chimiques
Sesquiterpenes
0
patchouli alcohol
HHH8CPR1M2
Mevalonic Acid
S5UOB36OCZ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1348-1356Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP18K14069
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : JP18KK0413
Pays : International
Informations de copyright
© 2020 Wiley Periodicals, Inc.
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