Variant antigen diversity in Trypanosoma vivax is not driven by recombination.

Antigenic Variation / genetics DNA, Protozoan Evolution, Molecular Genome, Protozoan Host-Parasite Interactions / immunology Immune Evasion Phylogeny Protozoan Proteins / genetics Recombination, Genetic / genetics Sequence Homology Species Specificity Transcriptome Trypanosoma brucei brucei / genetics Trypanosoma vivax / genetics Trypanosomiasis, African / immunology Variant Surface Glycoproteins, Trypanosoma / genetics

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
12 02 2020
Historique:
received: 30 08 2019
accepted: 18 01 2020
entrez: 14 2 2020
pubmed: 14 2 2020
medline: 24 4 2020
Statut: epublish

Résumé

African trypanosomes (Trypanosoma) are vector-borne haemoparasites that survive in the vertebrate bloodstream through antigenic variation of their Variant Surface Glycoprotein (VSG). Recombination, or rather segmented gene conversion, is fundamental in Trypanosoma brucei for both VSG gene switching and for generating antigenic diversity during infections. Trypanosoma vivax is a related, livestock pathogen whose VSG lack structures that facilitate gene conversion in T. brucei and mechanisms underlying its antigenic diversity are poorly understood. Here we show that species-wide VSG repertoire is broadly conserved across diverse T. vivax clinical strains and has limited antigenic repertoire. We use variant antigen profiling, coalescent approaches and experimental infections to show that recombination plays little role in diversifying T. vivax VSG sequences. These results have immediate consequences for both the current mechanistic model of antigenic variation in African trypanosomes and species differences in virulence and transmission, requiring reconsideration of the wider epidemiology of animal African trypanosomiasis.

Identifiants

DOI: 10.1038/s41467-020-14575-8 PMID: 32051413 PMC: PMC7015903
pubmed: 32051413
doi: 10.1038/s41467-020-14575-8
pii: 10.1038/s41467-020-14575-8
pmc: PMC7015903
doi:

Substances chimiques

DNA, Protozoan 0
Protozoan Proteins 0
Variant Surface Glycoproteins, Trypanosoma 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

844

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/M022811/1
Pays : International
Organisme : Wellcome Trust (Wellcome)
ID : WT206815/Z/17/Z
Pays : International
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/R021139/1
Pays : International

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Auteurs

Sara Silva Pereira (S)

Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, 146 Brownlow Hill, Liverpool, L3 5RF, UK.
ORCID: 0000-0002-6590-6626

Kayo J G de Almeida Castilho Neto (KJG)

Department of Veterinary Pathology, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.

Craig W Duffy (CW)

Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, 146 Brownlow Hill, Liverpool, L3 5RF, UK.
ORCID: 0000-0001-9359-4888

Peter Richards (P)

Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, 146 Brownlow Hill, Liverpool, L3 5RF, UK.

Harry Noyes (H)

Institute of Integrative Biology, University of Liverpool, Biosciences Building, Crown Street, Liverpool, L69 7ZB, UK.

Moses Ogugo (M)

Livestock Genetic Programme, International Livestock Research Institute, 30709 Naivasha Road, Nairobi, Kenya.

Marcos Rogério André (M)

Department of Veterinary Pathology, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.

Zakaria Bengaly (Z)

International Research Centre for Livestock Development in the Sub-humid Zone (CIRDES), No. 559, rue 5-31 angle, Avenue du Gouverneur Louveau, Bobo-Dioulasso, Burkina Faso.

Steve Kemp (S)

Livestock Genetic Programme, International Livestock Research Institute, 30709 Naivasha Road, Nairobi, Kenya.

Marta M G Teixeira (MMG)

Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, Avenue Professor Lineu Prestes, 1374 Cidade Universitaria, Sao Paulo, SP, 05508-000, Brazil.

Rosangela Z Machado (RZ)

Department of Veterinary Pathology, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.

Andrew P Jackson (AP)

Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, 146 Brownlow Hill, Liverpool, L3 5RF, UK. a.p.jackson@liv.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Phénomènes du système immunitaire › Phénomènes immunogénétiques › Variation des antigènes › Variant antigen diversity in Trypanosoma vivax...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN des protozoaires › Variant antigen diversity in Trypanosoma vivax...
questionsmedicales.fr › Phénomènes biologiques › Évolution biologique › Évolution moléculaire › Variant antigen diversity in Trypanosoma vivax...
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