A cell atlas of human thymic development defines T cell repertoire formation.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
21 02 2020
Historique:
received: 08 06 2019
accepted: 16 01 2020
entrez: 22 2 2020
pubmed: 23 2 2020
medline: 17 4 2020
Statut: ppublish

Résumé

The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα

Identifiants

pubmed: 32079746
pii: 367/6480/eaay3224
doi: 10.1126/science.aay3224
pmc: PMC7611066
mid: EMS127082
pii:
doi:

Substances chimiques

Receptors, Antigen, T-Cell 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S035842/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_12009
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 221052
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_17180
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R006237/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : European Research Council
ID : 646794
Pays : International

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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Auteurs

Jong-Eun Park (JE)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Rachel A Botting (RA)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Cecilia Domínguez Conde (C)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Dorin-Mirel Popescu (DM)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Marieke Lavaert (M)

Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium.
Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium.

Daniel J Kunz (DJ)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
Theory of Condensed Matter Group, Cavendish Laboratory/Department of Physics, University of Cambridge, Cambridge CB3 0HE, UK.
Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.

Issac Goh (I)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Emily Stephenson (E)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Roberta Ragazzini (R)

Epithelial Stem Cell Biology and Regenerative Medicine Laboratory, Francis Crick Institute, London NW1 1AT, UK.
Great Ormond Street Institute of Child Health, University College London, London, UK.

Elizabeth Tuck (E)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Anna Wilbrey-Clark (A)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Kenny Roberts (K)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Veronika R Kedlian (VR)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

John R Ferdinand (JR)

Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QQ, UK.

Xiaoling He (X)

John van Geest Centre for Brain Repair, University of Cambridge, Cambridge CB2 0PY, UK.

Simone Webb (S)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Daniel Maunder (D)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Niels Vandamme (N)

Data Mining and Modeling for Biomedicine, VIB Center for Inflammation Research, Ghent, Belgium.
Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium.

Krishnaa T Mahbubani (KT)

Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge CB2 0QQ, UK.

Krzysztof Polanski (K)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Lira Mamanova (L)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Liam Bolt (L)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

David Crossland (D)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
Department of Adult Congenital Heart Disease and Paediatric Cardiology/Cardiothoracic Surgery, Freeman Hospital, Newcastle Hospitals NHS Foundation Trust, Newcastle upon Tyne NE2 4LP, UK.

Fabrizio de Rita (F)

Department of Adult Congenital Heart Disease and Paediatric Cardiology/Cardiothoracic Surgery, Freeman Hospital, Newcastle Hospitals NHS Foundation Trust, Newcastle upon Tyne NE2 4LP, UK.

Andrew Fuller (A)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Andrew Filby (A)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Gary Reynolds (G)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

David Dixon (D)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Kourosh Saeb-Parsy (K)

Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge CB2 0QQ, UK.

Steven Lisgo (S)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Deborah Henderson (D)

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Roser Vento-Tormo (R)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Omer A Bayraktar (OA)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Roger A Barker (RA)

John van Geest Centre for Brain Repair, University of Cambridge, Cambridge CB2 0PY, UK.
WT-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre Cambridge Biomedical Campus, Cambridge CB2 0AW, UK.

Kerstin B Meyer (KB)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Yvan Saeys (Y)

Data Mining and Modeling for Biomedicine, VIB Center for Inflammation Research, Ghent, Belgium.
Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium.

Paola Bonfanti (P)

Epithelial Stem Cell Biology and Regenerative Medicine Laboratory, Francis Crick Institute, London NW1 1AT, UK.
Great Ormond Street Institute of Child Health, University College London, London, UK.
Institute of Immunity and Transplantation, University College London, London, UK.

Sam Behjati (S)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
Department of Paediatrics, University of Cambridge, Cambridge CB2 0SP, UK.

Menna R Clatworthy (MR)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QQ, UK.
Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK.

Tom Taghon (T)

Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, 9000 Ghent, Belgium. m.a.haniffa@newcastle.ac.uk tom.taghon@ugent.be st9@sanger.ac.uk.
Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium.

Muzlifah Haniffa (M)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK. m.a.haniffa@newcastle.ac.uk tom.taghon@ugent.be st9@sanger.ac.uk.
Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
Department of Dermatology and NIHR Newcastle Biomedical Research Centre, Newcastle Hospitals NHS Foundation Trust, Newcastle upon Tyne NE2 4LP, UK.

Sarah A Teichmann (SA)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK. m.a.haniffa@newcastle.ac.uk tom.taghon@ugent.be st9@sanger.ac.uk.
Theory of Condensed Matter Group, Cavendish Laboratory/Department of Physics, University of Cambridge, Cambridge CB3 0HE, UK.

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