Chromatin Analysis of Metabolic Gene Clusters in Plants.

Arabidopsis / genetics Arabidopsis Proteins / genetics Chromatin / genetics Gene Expression Regulation, Plant / genetics Genome, Plant / genetics Histones / genetics Metabolic Networks and Pathways / genetics Multigene Family / genetics
Chromatin modifications Gene cluster Metabolism Triterpene

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 24 2 2020
pubmed: 24 2 2020
medline: 30 12 2020
Statut: ppublish

Résumé

Plant metabolic gene clusters consist of neighboring genes that are involved in the biosynthesis of secondary or specialized metabolites. The genes within clusters are typically co-regulated, share a common set of chromatin marks, and code for the biosynthesis enzymes of a single metabolic pathway. Here, we describe three essential protocols for the basic analysis of metabolic gene clusters at transcription, histone modification, and metabolite level. The protocols are specified to clusters in the Arabidopsis thaliana genome and are transferable to other plant species.

Identifiants

DOI: 10.1007/978-1-0716-0179-2_10 PMID: 32088894
pubmed: 32088894
doi: 10.1007/978-1-0716-0179-2_10
doi:

Substances chimiques

Arabidopsis Proteins 0
Chromatin 0
Histones 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

129-146

Auteurs

Ancheng C Huang (AC)

Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, China. huangac@sustech.edu.cn.

Hans-Wilhelm Nützmann (HW)

The Milner Centre for Evolution, University of Bath, Bath, UK. h.nuetzmann@bath.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome végétal › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Chromatin Analysis of Metabolic Gene Clusters...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Famille multigénique › Chromatin Analysis of Metabolic Gene Clusters...