Membrane Protein Production in Escherichia coli.

Cloning, Molecular / methods Crystallography, X-Ray DNA Transformation Competence Escherichia coli / genetics Gene Expression Regulation, Bacterial Ion Transport / genetics Membrane Proteins / chemistry Membrane Transport Proteins / chemistry Organisms, Genetically Modified Phylogeny Recombinant Fusion Proteins / chemistry Recombinant Proteins / chemistry Transformation, Bacterial
Crystallography E. coli Ion channel Membrane protein Transporter.

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 1 3 2020
pubmed: 1 3 2020
medline: 9 3 2021
Statut: ppublish

Résumé

Escherichia coli is the workhorse of the structural biology lab. In addition to routine cloning and molecular biology, E. coli can be used as a factory for the production of recombinant membrane proteins. Purification of homogeneous samples of membrane protein expressed in E. coli is a significant bottleneck for researchers, and the protocol we present here for the overexpression and purification of membrane proteins in E. coli will provide a solid basis to develop lab- and protein-specific protocols for your membrane protein of interest. We additionally provide extensive notes on the purification process, as well as the theory surrounding principles of purification.

Identifiants

DOI: 10.1007/978-1-0716-0373-4_2 PMID: 32112312
pubmed: 32112312
doi: 10.1007/978-1-0716-0373-4_2
doi:

Substances chimiques

Membrane Proteins 0
Membrane Transport Proteins 0
Recombinant Fusion Proteins 0
Recombinant Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

13-27

Auteurs

Benjamin C McIlwain (BC)

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. mcilwain@umich.edu.

Ali A Kermani (AA)

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. kermania@umich.edu.

Articles similaires

Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.

Yue Zhang, Meifang Song, Deying Tang et al.
1.00
Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing

Decoding the genomic terrain: functional insights into 14 chemosensory proteins in whitefly Bemisia tabaci Asia II-1.

M N Rudra Gouda, Sabtharishi Subramanian
1.00
Animals Hemiptera Insect Proteins Phylogeny Insecticides

Multiple NADPH-cytochrome P450 reductases from Lycoris radiata involved in Amaryllidaceae alkaloids biosynthesis.

Yuqing Wu, Yifeng Zhang, Haitong Fan et al.
1.00
Amaryllidaceae Alkaloids Lycoris NADPH-Ferrihemoprotein Reductase Gene Expression Regulation, Plant Plant Proteins

Systematical characterization of Rab7 gene family in Gossypium and potential functions of GhRab7B3-A gene in drought tolerance.

Mengyuan Yan, Zhiwei Dong, Tian Pan et al.
1.00
Drought Resistance Gene Expression Profiling Gene Expression Regulation, Plant Gossypium Multigene Family

Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques bactériens › Transformation bactérienne › Compétence pour la transformation par l'ADN › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Métabolisme › Transport biologique › Transport des ions › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Membrane Protein Production in Escherichia coli.
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques bactériens › Transformation bactérienne › Membrane Protein Production in Escherichia coli.