A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de-etiolation in Zea mays.

Acetylation Etiolation Lysine / metabolism Metabolome Plant Proteins / metabolism Sunlight Zea mays / physiology
De-etiolation Lysine-acetylation Maize seedling Photosynthesis establishment

Journal

Journal of plant physiology
ISSN: 1618-1328
Titre abrégé: J Plant Physiol
Pays: Germany
ID NLM: 9882059

Informations de publication

Date de publication:
May 2020
Historique:
received: 16 12 2019
revised: 02 02 2020
accepted: 02 02 2020
pubmed: 3 4 2020
medline: 17 9 2020
entrez: 3 4 2020
Statut: ppublish

Résumé

Lysine acetylation is one of the most important post-translational modifications and is involved in multiple cellular processes in plants. There is evidence that acetylation may play an important role in light-induced de-etiolation, a key developmental switch from skotomorphogenesis to photomorphogenesis. During this transition, establishment of photosynthesis is of great significance. However, studies on acetylome dynamics during de-etiolation are limited. Here, we performed the first global lysine acetylome analysis for Zea mays seedlings undergoing de-etiolation, using nano liquid chromatography coupled to tandem mass spectrometry, and identified 814 lysine-acetylated sites on 462 proteins. Bioinformatics analysis of this acetylome showed that most of the lysine-acetylated proteins are predicted to be located in the cytoplasm, nucleus, chloroplast, and mitochondria. In addition, we detected ten lysine acetylation motifs and found that the accumulation of 482 lysine-acetylated peptides corresponding to 289 proteins changed significantly during de-etiolation. These proteins include transcription factors, histones, and proteins involved in chlorophyll synthesis, photosynthesis light reaction, carbon assimilation, glycolysis, the TCA cycle, amino acid metabolism, lipid metabolism, and nucleotide metabolism. Our study provides an in-depth dataset that extends our knowledge of in vivo acetylome dynamics during de-etiolation in monocots. This dataset promotes our understanding of the functional consequences of lysine acetylation in diverse cellular metabolic regulatory processes, and will be a useful toolkit for further investigations of the lysine acetylome and de-etiolation in plants.

Identifiants

DOI: 10.1016/j.jplph.2020.153158 PMID: 32240968
pubmed: 32240968
pii: S0176-1617(20)30047-X
doi: 10.1016/j.jplph.2020.153158
pii:
doi:

Substances chimiques

Plant Proteins 0
Lysine K3Z4F929H6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

153158

Informations de copyright

Copyright © 2020 Elsevier GmbH. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest All the authors have declared no competing interests.

Auteurs

Zhen Yan (Z)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: yanzhensky@ibcas.ac.cn.

Zhuo Shen (Z)

Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China. Electronic address: shenzhuo@gdaas.cn.

Zhi-Fang Gao (ZF)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: zfgao12@ibcas.ac.cn.

Qing Chao (Q)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100039, China. Electronic address: chaoqing@ibcas.ac.cn.

Chun-Rong Qian (CR)

Institute of Crop Cultivation and Farming, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China. Electronic address: qcr3906@163.com.

Haiyan Zheng (H)

Center for Advanced Biotechnology and Medicine, Biological Mass Spectrometry Facility, Rutgers University, Piscataway, New Jersey 08855, USA. Electronic address: haiyanz@cabm.rutgers.edu.

Bai-Chen Wang (BC)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100039, China. Electronic address: wangbc@ibcas.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Acylation › Acétylation › A comprehensive analysis of the lysine...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Développement des plantes › Étiolement › A comprehensive analysis of the lysine...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Lysine › A comprehensive analysis of the lysine...
questionsmedicales.fr › Métabolisme › Métabolome › A comprehensive analysis of the lysine...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › A comprehensive analysis of the lysine...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Lumière du soleil › A comprehensive analysis of the lysine...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Zea mays › A comprehensive analysis of the lysine...