SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
Adult
Aging
Angiotensin-Converting Enzyme 2
Bronchi
/ cytology
COVID-19
Cells, Cultured
Chronic Disease
/ epidemiology
Coronavirus Infections
/ genetics
Epithelial Cells
/ metabolism
Female
Gene Expression
Gene Expression Profiling
Germany
Goblet Cells
/ metabolism
Humans
Lung
/ cytology
Male
Middle Aged
Pandemics
Peptidyl-Dipeptidase A
/ genetics
Pneumonia, Viral
/ genetics
Reference Standards
Sequence Analysis, RNA
Serine Endopeptidases
/ genetics
Sex Characteristics
Single-Cell Analysis
Smoking
Tissue Banks
FURIN
COVID-19
Human Cell Atlas
epithelial differentiation
respiratory tract
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
18 05 2020
18 05 2020
Historique:
received:
27
03
2020
accepted:
30
03
2020
pubmed:
5
4
2020
medline:
21
5
2020
entrez:
5
4
2020
Statut:
ppublish
Résumé
The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.
Identifiants
pubmed: 32246845
doi: 10.15252/embj.20105114
pmc: PMC7232010
doi:
Substances chimiques
Peptidyl-Dipeptidase A
EC 3.4.15.1
ACE2 protein, human
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Serine Endopeptidases
EC 3.4.21.-
TMPRSS2 protein, human
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e105114Subventions
Organisme : EC|Horizon 2020 Framework Programme (H2020)
ID : 874710
Pays : International
Organisme : German Center for Lung Research (DLZ)
ID : 82DZL00402
Pays : International
Organisme : European Respiratory Society (STRTF - 201804-00377) - fellowship CV
Pays : International
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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