Residues in Transmembrane Segments of the P2X4 Receptor Contribute to Channel Function and Ethanol Sensitivity.
TM1 and TM2 segments
ethanol and agonist action
molecular model
mutagenesis
purinergic (P2X4) receptor
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Apr 2020
02 Apr 2020
Historique:
received:
04
03
2020
revised:
28
03
2020
accepted:
31
03
2020
entrez:
8
4
2020
pubmed:
8
4
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Mouse models of alcohol use disorder (AUD) revealed purinergic P2X4 receptors (P2X4Rs) as a promising target for AUD drug development. We have previously demonstrated that residues at the transmembrane (TM)-ectodomain interface and within the TM1 segment contribute to the formation of an ethanol action pocket in P2X4Rs. In the present study, we tested the hypothesis that there are more residues in TM1 and TM2 segments that are important for the ethanol sensitivity of P2X4Rs. Using site-directed mutagenesis and two electrode voltage-clamp electrophysiology in
Identifiants
pubmed: 32252459
pii: ijms21072471
doi: 10.3390/ijms21072471
pmc: PMC7178174
pii:
doi:
Substances chimiques
Amino Acids
0
Purinergic P2X Receptor Agonists
0
Receptors, Purinergic P2X4
0
Ethanol
3K9958V90M
Arginine
94ZLA3W45F
Alanine
OF5P57N2ZX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Pflugers Arch. 2008 Aug;456(5):939-50
pubmed: 18427835
J Neurochem. 2008 Aug;106(3):1337-49
pubmed: 18485105
FEBS Lett. 1992 Oct 26;311(3):217-20
pubmed: 1397317
J Gen Physiol. 2005 Apr;125(4):347-59
pubmed: 15795310
Br J Pharmacol. 2000 Jul;130(6):1394-8
pubmed: 10903981
Cell Mol Neurobiol. 2010 Nov;30(8):1251-8
pubmed: 21107680
Nature. 2012 May 10;485(7397):207-12
pubmed: 22535247
J Biol Chem. 2012 Mar 2;287(10):7594-602
pubmed: 22219189
J Biol Chem. 2008 Sep 19;283(38):26241-51
pubmed: 18635539
Eur J Neurosci. 1996 Oct;8(10):2221-8
pubmed: 8921315
J Neurosci. 2009 Nov 11;29(45):14257-64
pubmed: 19906973
Neuropsychopharmacology. 2013 Sep;38(10):1993-2002
pubmed: 23604007
Front Neurosci. 2014 Jun 24;8:176
pubmed: 25009459
J Biol Chem. 2001 May 4;276(18):14902-8
pubmed: 11278888
Mol Pharmacol. 2014 Dec;86(6):635-46
pubmed: 25245406
Neuron. 2007 Apr 19;54(2):263-74
pubmed: 17442247
BMC Biol. 2009 Oct 27;7:70
pubmed: 19874574
J Neurosci. 1998 Apr 1;18(7):2350-9
pubmed: 9502796
Alcohol. 2007 Mar;41(2):95-132
pubmed: 17517326
J Neurochem. 2009 May;109(3):923-34
pubmed: 19425179
J Biol Chem. 2010 Mar 26;285(13):10110-21
pubmed: 20093367
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3684-8
pubmed: 8622997
Brain Res. 1998 Jun 15;796(1-2):53-62
pubmed: 9689454
Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):E4045-54
pubmed: 24082111
Pharmacol Ther. 2004 Aug;103(2):121-46
pubmed: 15369680
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11648-52
pubmed: 8265602
J Pharmacol Exp Ther. 2011 Apr;337(1):171-9
pubmed: 21212160
J Neurochem. 2007 Sep;102(6):2097-2109
pubmed: 17561937
Purinergic Signal. 2013 Dec;9(4):621-32
pubmed: 23817978
J Biol Chem. 2001 Aug 31;276(35):32793-8
pubmed: 11438537
Biopolymers. 1992 Apr;32(4):399-406
pubmed: 1623135
J Neurochem. 2010 Jan;112(1):307-17
pubmed: 19878433
Neuropharmacology. 2005 Aug;49(2):243-53
pubmed: 15993446
Nature. 2009 Jul 30;460(7255):592-8
pubmed: 19641588
Am J Prev Med. 2015 Nov;49(5):e73-e79
pubmed: 26477807
J Pharmacol Exp Ther. 2010 Sep 1;334(3):720-8
pubmed: 20543096