Single‑cell RNA sequencing of t(8;21) acute myeloid leukemia for risk prediction.
Adult
Aged
Biomarkers, Tumor
/ genetics
Chromosomes, Human, Pair 21
/ genetics
Chromosomes, Human, Pair 8
/ genetics
Female
Gene Expression Regulation, Leukemic
Heterogeneous-Nuclear Ribonucleoproteins
/ genetics
Histone-Lysine N-Methyltransferase
/ genetics
Humans
Leukemia, Myeloid, Acute
/ genetics
Middle Aged
Myeloid-Lymphoid Leukemia Protein
/ genetics
Neoplasm Recurrence, Local
/ genetics
Prognosis
Risk Factors
Sequence Analysis, RNA
/ methods
Single-Cell Analysis
/ methods
Transcription Factors
/ genetics
Translocation, Genetic
acute myeloid leukemia
single-cell RNA-sequencing
prognosis
Journal
Oncology reports
ISSN: 1791-2431
Titre abrégé: Oncol Rep
Pays: Greece
ID NLM: 9422756
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
11
09
2019
accepted:
22
01
2020
pubmed:
24
4
2020
medline:
17
12
2020
entrez:
24
4
2020
Statut:
ppublish
Résumé
Single‑cell RNA sequencing (scRNA‑seq) of bone marrow or peripheral blood samples from patients with acute myeloid leukemia (AML) enables the characterization of heterogeneous malignant cells. A total of 87 cells from two patients with t(8;21) AML were analyzed using scRNA‑seq. Clustering methods were used to separate leukemia cells into different sub‑populations, and the expression patterns of specific marker genes were used to annotate these populations. Among the 31 differentially expressed genes in the cells of a patient who relapsed after hematopoietic stem cell transplantation, 13 genes were identified to be associated with leukemia. Furthermore, three genes, namely AT‑rich interaction domain 2, lysine methyltransferase 2A and synaptotagmin binding cytoplasmic RNA interacting protein were validated as possible prognostic biomarkers using two bulk expression datasets. Taking advantage of scRNA‑seq, the results of the present study may provide clinicians with several possible biomarkers to predict the prognostic outcomes of t(8;21) AML.
Identifiants
pubmed: 32323795
doi: 10.3892/or.2020.7507
pmc: PMC7057921
doi:
Substances chimiques
ARID2 protein, human
0
Biomarkers, Tumor
0
Heterogeneous-Nuclear Ribonucleoproteins
0
KMT2A protein, human
0
SYNCRIP protein, human
0
Transcription Factors
0
Myeloid-Lymphoid Leukemia Protein
149025-06-9
Histone-Lysine N-Methyltransferase
EC 2.1.1.43
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1278-1288Références
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