High-Throughput DNA Sequence-Based Analysis of AMF Communities.
Arbuscular mycorrhizal fungi (AMF)
DNA-based species characterization
High-throughput sequence
ITS primers
Illumina MiSeq
SSU primers
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
17
5
2020
pubmed:
18
5
2020
medline:
16
3
2021
Statut:
ppublish
Résumé
Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ecological and economic impacts as they support plant nutrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by high-throughput sequencing through Illumina MiSeq platform of two DNA target regions: small subunit (SSU) and internal transcribed spacer (ITS). The protocol can apply to both soil and root AMF communities.
Identifiants
pubmed: 32415599
doi: 10.1007/978-1-0716-0603-2_9
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM