Kinetic and thermodynamic study of c-Met interaction with single chain fragment variable (scFv) antibodies using phage based surface plasmon resonance.
c-Met
cancer
scFv
Journal
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
ISSN: 1879-0720
Titre abrégé: Eur J Pharm Sci
Pays: Netherlands
ID NLM: 9317982
Informations de publication
Date de publication:
01 Jul 2020
01 Jul 2020
Historique:
received:
07
12
2019
revised:
19
03
2020
accepted:
23
04
2020
pubmed:
18
5
2020
medline:
23
3
2021
entrez:
17
5
2020
Statut:
ppublish
Résumé
Mesenchymal epithelial transition factor (c-Met) has been recently regarded as an attractive target for the treatment of cancer. Our previous study showed that c-Met-specific single chain fragment variables (scFvs) can be considered as a promising therapy for cancer, however, their molecular interaction with c-Met protein have not been assessed. Accordingly, in the current study we aim to evaluate the kinetic and thermodynamic properties of c-Met interaction with these scFvs as anticancer agents by means of surface plasmon resonance (SPR) technique. Phage-scFvs were immobilized on the 11-mercaptoundecanoic acid gold chips after carboxylic groups activation by N-ethyl-N-(3-diethylaminopropyl) carbodiimide/N-hydroxysuccinimide and, then the c-Met binding to each scFvs (ES1, ES2, and ES3) at different concentrations (ranging from 20 to 665 μM) was explored. Kinetic studies revealed that ES1 has the highest affinity (K
Identifiants
pubmed: 32416255
pii: S0928-0987(20)30151-2
doi: 10.1016/j.ejps.2020.105362
pii:
doi:
Substances chimiques
11-mercaptoundecanoic acid
0
Carbodiimides
0
Fatty Acids
0
Single-Chain Antibodies
0
Succinimides
0
Sulfhydryl Compounds
0
Gold
7440-57-5
Proto-Oncogene Proteins c-met
EC 2.7.10.1
N-hydroxysuccinimide
MJE3791M4T
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105362Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None