Genomic analysis of fluoroquinolone-susceptible phylogenetic group B2 extraintestinal pathogenic Escherichia coli causing infections in cats.
Animals
Anti-Bacterial Agents
/ pharmacology
Bacteremia
/ microbiology
Cat Diseases
/ microbiology
Cats
/ microbiology
Escherichia coli Infections
/ veterinary
Extraintestinal Pathogenic Escherichia coli
/ classification
Fluoroquinolones
/ pharmacology
Genomics
Genotype
Humans
Phylogeny
Urinary Tract Infections
/ microbiology
Virulence
/ genetics
Whole Genome Sequencing
Companion animals
Escherichia coli
Genomics
Virulence genes
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
04
12
2019
revised:
08
04
2020
accepted:
08
04
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
2
2
2021
Statut:
ppublish
Résumé
Extraintestinal pathogenic Escherichia coli (ExPEC) can cause urinary tract and other types of infection in cats, but the relationship of cat ExPEC to human ExPEC remains equivocal. This study investigated the prevalence of ExPEC-associated sequence types (STs) from phylogenetic group B2 among fluoroquinolone-susceptible cat clinical isolates. For this, 323 fluoroquinolone-susceptible cat clinical E. coli isolates from Australia underwent PCR-based phylotyping and random amplified polymorphic DNA analysis to determine clonal relatedness. Of the 274 group B2 isolates, 53 underwent whole genome sequencing (WGS), whereas 221 underwent PCR-based screening for (group B2) sequence type complexes (STc) STc12, STc73, ST131, and STc372. Group B2 was the dominant phylogenetic group (274/323, 85 %), whereas within group B2 ST73 dominated, according to both WGS (43 % of 53; followed by ST127, ST12, and ST372 [4/53, 8 % each]) and ST-specific PCR (20 % of 221). In WGS-based comparisons of cat and reference human ST73 isolates, cat isolates had a relatively conserved virulence gene profile but were phylogenetically diverse. Although in the phylogram most cat and human ST73 isolates occupied host species-specific clusters within serotype-specific clades (O2:H1, O6:H1, O25:H1, O50/O2:H1), cat and human isolates were intermingled within two serotype-specific clades: O120:H31 (3 cat and 2 human isolates) and O22:H1 (3 cat and 5 human isolates). These findings confirm the importance of human-associated group B2 lineages as a cause of urinary tract infections in cats. The close genetic relationship of some cat and human ST73 strains suggests bi-directional transmission may be possible.
Identifiants
pubmed: 32456818
pii: S0378-1135(19)31406-3
doi: 10.1016/j.vetmic.2020.108685
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Fluoroquinolones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108685Subventions
Organisme : CSRD VA
ID : I01 CX000920
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest DJT has received research funding and undertaken consultancies for Bayer, Zoetis, Boehringer Ingelheim, Virbac, Luoda Pharma, Neoculi and IRiccorgpharm. SA has received research funding from Zoetis and Neoculi. JRJ has received research support from and/or has undertaken consultancies for Achaogen, Allergan, Crucell/Janssen, Melinta, Merck, Shionogi, Syntiron, and Tetraphase.