Crucial role of leaky initiation of uORF3 in the downregulation of HNT1 by ER stress.
Leaky initiation
Translational control
Unfolded protein response (UPR)
uORF
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
12 07 2020
12 07 2020
Historique:
received:
31
03
2020
accepted:
20
04
2020
pubmed:
2
6
2020
medline:
14
1
2021
entrez:
2
6
2020
Statut:
ppublish
Résumé
eIF2α phosphorylation-mediated translational regulation is crucial for global repression of translation by various stresses, including the unfolded protein response (UPR) in eukaryotes. Although translational control during UPR has not been extensively investigated in S. cerevisiae, Hac1-mediated production of long transcripts containing uORFs was shown to repress the translation of histidine triad nucleotide-binding 1 (HNT1) mRNA. The present study showed that uORF3 is required for HNT1 expression, as well as down-regulating HNT1 translation. Translation initiation by uORF3 is inefficient, with uORF3 having a strong Kozak sequence efficiently repressing the translation of HNT1. We propose that leaky scanning of uORF3 is responsible for the downregulation of HNT1 during UPR.
Identifiants
pubmed: 32475637
pii: S0006-291X(20)30832-9
doi: 10.1016/j.bbrc.2020.04.104
pii:
doi:
Substances chimiques
5' Untranslated Regions
0
Saccharomyces cerevisiae Proteins
0
histidine triad protein
0
Hydrolases
EC 3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
186-192Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.