Boron inhibits cadmium uptake in wheat (Triticum aestivum) by regulating gene expression.
Analysis
Boron
Cadmium
Gene expression
RNA-Seq
Uptake
Wheat
Journal
Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
10
02
2020
revised:
30
04
2020
accepted:
04
05
2020
entrez:
22
6
2020
pubmed:
22
6
2020
medline:
28
1
2021
Statut:
ppublish
Résumé
Various nutrients (Mg, Zn, Fe, Mn, Si, etc.) can supress cadmium (Cd) uptake and alleviate Cd toxicity, but the mechanisms are not the same. In this study, the molecular mechanism governing the effects of boron (B) on uptake of Cd in hydroponically grown wheat was characterized. As compared to control (0 μM Cd), B concentration per plant decreased by 22% and 29% under 5 μM Cd and 50 μM Cd treatment respectively. In addition, B application decreased Cd concentration and accumulation in whole wheat. Correlation analysis of different elements show that there was a highly negative correlation between concentrations of B and Cd (r = -0.854 with significant correlation) in wheat. Additionally, 16,543 differentially expressed genes (DEGs) (7666 up- and 8877 down-regulated) were detected between 0 and 5 μM Cd treatments in wheat roots by transcriptome sequencing. Gene ontology functional category and Kyoto encyclopedia of genes and genomes pathway analyses indicated that the DEGs were involved in biological process, cellular component, and molecular function. Five highly homologous genes to Cd transporters were identified; these genes were involved in metal ion binding, transmembrane ion transport, and protein transport. According to the qRT-PCR results, expression of all these genes was down-regulated in the 462 μM of B treatment compared with the 46.2 μM of B treatment regardless of the Cd treatments (0.5 or 5 μM Cd). These results suggest that B is an inhibitor of Cd uptake, and the down-regulation of five highly homologous genes could be associated with decreased uptake of Cd after B application.
Identifiants
pubmed: 32563461
pii: S0168-9452(20)30127-8
doi: 10.1016/j.plantsci.2020.110522
pii:
doi:
Substances chimiques
Membrane Transport Proteins
0
Plant Proteins
0
Cadmium
00BH33GNGH
Boron
N9E3X5056Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110522Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted, the manuscript entitled, “Boron inhibits cadmium uptake in wheat (Triticum aestivum) by regulating gene expression”