Changes in the host transcriptome and microbial metatranscriptome of the ileum of dairy calves subjected to artificial dosing of exogenous rumen contents.
Animal Feed
/ microbiology
Animals
Animals, Newborn
Body Fluids
/ microbiology
Cattle
/ genetics
Female
Gastrointestinal Microbiome
/ genetics
Gene Ontology
Genes
Host Microbial Interactions
/ genetics
Ileum
/ microbiology
Immunity, Innate
/ genetics
Male
RNA, Ribosomal
/ genetics
RNA-Seq
/ methods
Rumen
/ microbiology
Transcriptome
Weaning
artificial dosing
calf
ileum tissue
microbial meta-transcriptome
transcriptome
Journal
Physiological genomics
ISSN: 1531-2267
Titre abrégé: Physiol Genomics
Pays: United States
ID NLM: 9815683
Informations de publication
Date de publication:
01 08 2020
01 08 2020
Historique:
pubmed:
23
6
2020
medline:
27
5
2021
entrez:
23
6
2020
Statut:
ppublish
Résumé
Development of a properly functioning gastrointestinal tract (GIT) at an early age is critical for the wellbeing and lifetime productivity of dairy cattle. The role of early microbial colonization on GIT development in neonatal cattle and the associated molecular changes remain largely unknown, particularly for the small intestine. In this study, we performed artificial dosing of exogenous rumen fluid during the early life of the calf, starting at birth through the weaning transition at 8 wk. Six calves were included in this study. At 8 wk of age, tissue from the ileum was collected and subjected to host transcriptome and microbial metatranscriptome analysis using RNA sequencing. A total of 333 genes showed significant differential expression (DE) (fold-change ≥2; adjusted
Identifiants
pubmed: 32567508
doi: 10.1152/physiolgenomics.00005.2020
doi:
Substances chimiques
RNA, Ribosomal
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM