Gene expression predictions and networks in natural populations supports the omnigenic theory.


Journal

BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258

Informations de publication

Date de publication:
22 Jun 2020
Historique:
received: 10 12 2019
accepted: 08 06 2020
entrez: 24 6 2020
pubmed: 24 6 2020
medline: 30 1 2021
Statut: epublish

Résumé

Recent literature on the differential role of genes within networks distinguishes core from peripheral genes. If previous works have shown contrasting features between them, whether such categorization matters for phenotype prediction remains to be studied. We measured 17 phenotypic traits for 241 cloned genotypes from a Populus nigra collection, covering growth, phenology, chemical and physical properties. We also sequenced RNA for each genotype and built co-expression networks to define core and peripheral genes. We found that cores were more differentiated between populations than peripherals while being less variable, suggesting that they have been constrained through potentially divergent selection. We also showed that while cores were overrepresented in a subset of genes statistically selected for their capacity to predict the phenotypes (by Boruta algorithm), they did not systematically predict better than peripherals or even random genes. Our work is the first attempt to assess the importance of co-expression network connectivity in phenotype prediction. While highly connected core genes appear to be important, they do not bear enough information to systematically predict better quantitative traits than other gene sets.

Sections du résumé

BACKGROUND BACKGROUND
Recent literature on the differential role of genes within networks distinguishes core from peripheral genes. If previous works have shown contrasting features between them, whether such categorization matters for phenotype prediction remains to be studied.
RESULTS RESULTS
We measured 17 phenotypic traits for 241 cloned genotypes from a Populus nigra collection, covering growth, phenology, chemical and physical properties. We also sequenced RNA for each genotype and built co-expression networks to define core and peripheral genes. We found that cores were more differentiated between populations than peripherals while being less variable, suggesting that they have been constrained through potentially divergent selection. We also showed that while cores were overrepresented in a subset of genes statistically selected for their capacity to predict the phenotypes (by Boruta algorithm), they did not systematically predict better than peripherals or even random genes.
CONCLUSION CONCLUSIONS
Our work is the first attempt to assess the importance of co-expression network connectivity in phenotype prediction. While highly connected core genes appear to be important, they do not bear enough information to systematically predict better quantitative traits than other gene sets.

Identifiants

pubmed: 32571208
doi: 10.1186/s12864-020-06809-2
pii: 10.1186/s12864-020-06809-2
pmc: PMC7310122
doi:

Substances chimiques

Plant Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

416

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Auteurs

Aurélien Chateigner (A)

BioForA, INRAE, ONF, Orléans, France.

Marie-Claude Lesage-Descauses (MC)

BioForA, INRAE, ONF, Orléans, France.

Odile Rogier (O)

BioForA, INRAE, ONF, Orléans, France.

Véronique Jorge (V)

BioForA, INRAE, ONF, Orléans, France.

Jean-Charles Leplé (JC)

BIOGECO, INRAE, Univ. Bordeaux, Cestas, France.

Véronique Brunaud (V)

Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Sud, Université d'Evry, Université Paris-Saclay, Gif sur Yvette, France.
Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Diderot, Sorbonne Paris-Cité, Gif sur Yvette, France.

Christine Paysant-Le Roux (CP)

Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Sud, Université d'Evry, Université Paris-Saclay, Gif sur Yvette, France.
Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Diderot, Sorbonne Paris-Cité, Gif sur Yvette, France.

Ludivine Soubigou-Taconnat (L)

Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Sud, Université d'Evry, Université Paris-Saclay, Gif sur Yvette, France.
Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Diderot, Sorbonne Paris-Cité, Gif sur Yvette, France.

Marie-Laure Martin-Magniette (ML)

Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Sud, Université d'Evry, Université Paris-Saclay, Gif sur Yvette, France.
Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Diderot, Sorbonne Paris-Cité, Gif sur Yvette, France.
MIA-Paris, AgroParisTech, INRAE, Paris, France.

Leopoldo Sanchez (L)

BioForA, INRAE, ONF, Orléans, France.

Vincent Segura (V)

BioForA, INRAE, ONF, Orléans, France. vincent.segura@inrae.fr.
AGAP, Université Montpellier, CIRAD, INRAE, Montpellier SupAgro, Montpellier, France. vincent.segura@inrae.fr.

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Classifications MeSH