The mutREAD method detects mutational signatures from low quantities of cancer DNA.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 06 2020
23 06 2020
Historique:
received:
11
01
2020
accepted:
03
06
2020
entrez:
25
6
2020
pubmed:
25
6
2020
medline:
28
8
2020
Statut:
epublish
Résumé
Mutational processes acting on cancer genomes can be traced by investigating mutational signatures. Because high sequencing costs limit current studies to small numbers of good-quality samples, we propose a robust, cost- and time-effective method, called mutREAD, to detect mutational signatures from small quantities of DNA, including degraded samples. We show that mutREAD recapitulates mutational signatures identified by whole genome sequencing, and will ultimately allow the study of mutational signatures in larger cohorts and, by compatibility with formalin-fixed paraffin-embedded samples, in clinical settings.
Identifiants
pubmed: 32576827
doi: 10.1038/s41467-020-16974-3
pii: 10.1038/s41467-020-16974-3
pmc: PMC7311535
doi:
Substances chimiques
DNA Primers
0
DNA, Neoplasm
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3166Subventions
Organisme : Medical Research Council
ID : MC_UU_12022/2
Pays : United Kingdom
Organisme : Cancer Research UK
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Organisme : Medical Research Council
ID : RG84369
Pays : United Kingdom
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pubmed: 26163694
pmcid: 4817139