Distinct follicular and luteal transcriptional profiles in engineered human ectocervical tissue dependent on menstrual cycle phase.
Adult
Cell Adhesion
Cell Proliferation
Cervix Uteri
/ cytology
Cluster Analysis
Epithelium
/ metabolism
Female
Follicular Phase
/ physiology
Gene Expression Regulation
/ genetics
High-Throughput Nucleotide Sequencing
Hormones
/ pharmacology
Humans
Luteal Phase
/ physiology
Menstrual Cycle
/ physiology
Models, Anatomic
Mucins
/ physiology
Tissue Engineering
3D model
differentiation
ectocervix
female reproductive tract
hormone action
mucins
Journal
Biology of reproduction
ISSN: 1529-7268
Titre abrégé: Biol Reprod
Pays: United States
ID NLM: 0207224
Informations de publication
Date de publication:
21 08 2020
21 08 2020
Historique:
received:
05
12
2018
revised:
24
04
2019
pubmed:
3
7
2020
medline:
12
10
2021
entrez:
3
7
2020
Statut:
ppublish
Résumé
To investigate genomic pathways that may influence physiology and infectivity during the menstrual cycle, RNA sequence analysis was performed on patient-matched engineered ectocervical tissue after follicular and luteal phase (LP) hormone treatments. We developed distinct cellular, molecular, and biological profiles in ectocervical epithelium dependent on the menstrual cycle phase. Follicular phase hormones were associated with proliferation, transcription, and cell adhesion, while LP samples expressed genes involved in immune cell recruitment, inflammation, and protein modifications. Additionally, our analysis revealed mucins not previously reported in ectocervical tissue, which could play an important role in fertility and disease prevention. This study provides insight into the phenomenon of increased LP vulnerability to infection and identifies potential targets for future research.
Identifiants
pubmed: 32614039
pii: 5823253
doi: 10.1093/biolre/ioaa056
pmc: PMC7822709
doi:
Substances chimiques
Hormones
0
Mucins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
487-496Subventions
Organisme : NCI NIH HHS
ID : T32 CA009560
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR001207
Pays : United States
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Références
Am J Reprod Immunol. 2015 Oct;74(4):357-67
pubmed: 26202107
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Mucosal Immunol. 2011 May;4(3):335-42
pubmed: 21048705
Clin Exp Metastasis. 2010 Apr;27(4):251-9
pubmed: 20364301
Reprod Sci. 2015 Aug;22(8):980-90
pubmed: 25676577
Nat Biotechnol. 2015 Mar;33(3):290-5
pubmed: 25690850
Nucleic Acids Res. 2015 Jul 1;43(W1):W566-70
pubmed: 25969447
PLoS One. 2015 Mar 23;10(3):e0121135
pubmed: 25798593
AIDS Res Hum Retroviruses. 2016 Jun;32(6):547-60
pubmed: 26750085
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
Nat Biotechnol. 2015 Mar;33(3):243-6
pubmed: 25748911
Biol Reprod. 2020 Aug 21;103(3):497-507
pubmed: 32401296
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
J Reprod Immunol. 2011 Mar;88(2):185-94
pubmed: 21353708
Cancer Res. 2016 Apr 1;76(7):1965-74
pubmed: 26880801
Biol Reprod. 1997 Apr;56(4):999-1011
pubmed: 9096884
Nucleic Acids Res. 2017 Jul 3;45(W1):W130-W137
pubmed: 28472511