DNA methylation reprogramming during seed development and its functional relevance in seed size/weight determination in chickpea.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
03 07 2020
03 07 2020
Historique:
received:
21
11
2019
accepted:
08
06
2020
entrez:
5
7
2020
pubmed:
6
7
2020
medline:
16
6
2021
Statut:
epublish
Résumé
Seed development is orchestrated via complex gene regulatory networks and pathways. Epigenetic factors may also govern seed development and seed size/weight. Here, we analyzed DNA methylation in a large-seeded chickpea cultivar (JGK 3) during seed development stages. Progressive gain of CHH context DNA methylation in transposable elements (TEs) and higher frequency of small RNAs in hypermethylated TEs during seed development suggested a role of the RNA-dependent DNA methylation pathway. Frequency of intragenic TEs was higher in CHH context differentially methylated region (DMR) associated differentially expressed genes (DEGs). CG context hyper/hypomethylation within the gene body was observed for most of DMR-associated DEGs in JGK 3 as compared to small-seeded chickpea cultivar (Himchana 1). We identified candidate genes involved in seed size/weight determination exhibiting CG context hypermethylation within the gene body and higher expression in JGK 3. This study provides insights into the role of DNA methylation in seed development and seed size/weight determination in chickpea.
Identifiants
pubmed: 32620865
doi: 10.1038/s42003-020-1059-1
pii: 10.1038/s42003-020-1059-1
pmc: PMC7335156
doi:
Substances chimiques
DNA Transposable Elements
0
DNA, Plant
0
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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