Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
21 07 2020
21 07 2020
Historique:
pubmed:
7
7
2020
medline:
24
2
2021
entrez:
7
7
2020
Statut:
ppublish
Résumé
Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence coverage when applied to
Identifiants
pubmed: 32628831
doi: 10.1021/acs.analchem.0c00478
pmc: PMC7377536
doi:
Substances chimiques
Proteins
0
Trypsin
EC 3.4.21.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9523-9527Subventions
Organisme : Wellcome Trust
ID : 103139
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108504
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203149
Pays : United Kingdom
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