Lactate production by Staphylococcus aureus biofilm inhibits HDAC11 to reprogramme the host immune response during persistent infection.
Biofilms
Biomarkers
Biosynthetic Pathways
Cytokines
/ metabolism
Histone Deacetylases
/ metabolism
Host-Pathogen Interactions
/ immunology
Inflammation Mediators
/ metabolism
Lactic Acid
/ metabolism
Macrophages
/ immunology
Myeloid-Derived Suppressor Cells
/ immunology
Staphylococcal Infections
/ immunology
Staphylococcus aureus
/ physiology
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
12
08
2019
accepted:
16
06
2020
pubmed:
15
7
2020
medline:
22
12
2020
entrez:
15
7
2020
Statut:
ppublish
Résumé
Staphylococcus aureus is a leading cause of biofilm-associated prosthetic joint infection (PJI), resulting in considerable disability and prolonged treatment. It is known that host leukocyte IL-10 production is required for S. aureus biofilm persistence in PJI. An S. aureus bursa aurealis Tn library consisting of 1,952 non-essential genes was screened for mutants that failed to induce IL-10 in myeloid-derived suppressor cells (MDSCs), which identified a critical role for bacterial lactic acid biosynthesis. We generated an S. aureus ddh/ldh1/ldh2 triple Tn mutant that cannot produce D- or L-lactate. Co-culture of MDSCs or macrophages with ddh/ldh1/ldh2 mutant biofilm produced substantially less IL-10 compared with wild-type S. aureus, which was also observed in a mouse model of PJI and led to reduced biofilm burden. Using MDSCs recovered from the mouse PJI model and in vitro leukocyte-biofilm co-cultures, we show that bacterial-derived lactate inhibits histone deacetylase 11, causing unchecked HDAC6 activity and increased histone 3 acetylation at the Il-10 promoter, resulting in enhanced Il-10 transcription in MDSCs and macrophages. Finally, we show that synovial fluid of patients with PJI contains elevated amounts of D-lactate and IL-10 compared with control subjects, and bacterial lactate increases IL-10 production by human monocyte-derived macrophages.
Identifiants
pubmed: 32661313
doi: 10.1038/s41564-020-0756-3
pii: 10.1038/s41564-020-0756-3
pmc: PMC7529909
mid: NIHMS1604938
doi:
Substances chimiques
Biomarkers
0
Cytokines
0
Inflammation Mediators
0
Lactic Acid
33X04XA5AT
HDAC11 protein, human
EC 3.5.1.98
Histone Deacetylases
EC 3.5.1.98
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1271-1284Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM110768
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA036727
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125588
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI083211
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103427
Pays : United States
Commentaires et corrections
Type : CommentIn
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