Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy.
CFPS
Cell-free synthesis
GFP
IDP
Isotope labelling
S12 extract
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
23
7
2020
pubmed:
23
7
2020
medline:
11
3
2021
Statut:
ppublish
Résumé
Cell-free protein synthesis (CFPS) is an established method to produce recombinant proteins and has been used in a wide variety of applications. The use of CFPS has almost from the onset been favorably linked to the production of isotopically labelled proteins for NMR spectroscopy as the resulting labelling of the produced protein is defined by the chosen amino acids during reaction setup. Here we describe how to set up production and isotopic labelling of small intrinsically disordered proteins (IDPs) for NMR spectroscopy applications using an E. coli-based CFPS system in batch mode.
Identifiants
pubmed: 32696360
doi: 10.1007/978-1-0716-0524-0_11
doi:
Substances chimiques
Intrinsically Disordered Proteins
0
Magnesium
I38ZP9992A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
233-245Références
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