Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control.
4-Butyrolactone
/ analogs & derivatives
Agrobacterium tumefaciens
/ metabolism
Bacterial Proteins
/ genetics
Gene Expression Regulation, Bacterial
/ drug effects
Homoserine
/ analogs & derivatives
Lactones
/ metabolism
Mutagenesis
Plasmids
/ genetics
Promoter Regions, Genetic
Pseudomonas aeruginosa
/ metabolism
Quorum Sensing
/ genetics
Trans-Activators
/ genetics
automatic delayed cascade control
promoter orthogonality
quorum sensing system
signal orthogonality
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
18 09 2020
18 09 2020
Historique:
pubmed:
14
8
2020
medline:
11
8
2021
entrez:
14
8
2020
Statut:
ppublish
Résumé
The existence of crosstalk between quorum sensing systems limits their application in a complex environment. In this study, two completely orthogonal quorum sensing systems with self-produced autoinducers were built in one cell to enable the systems to be signal orthogonal and promoter orthogonal to each other. The systems were designed on the basis of the las system from
Identifiants
pubmed: 32786361
doi: 10.1021/acssynbio.0c00370
doi:
Substances chimiques
Bacterial Proteins
0
Lactones
0
LasR protein, Pseudomonas aeruginosa
0
N-octanoylhomoserine lactone
0
TraR protein, Agrobacterium tumefaciens
0
Trans-Activators
0
Homoserine
6KA95X0IVO
N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone
76924-95-3
4-Butyrolactone
OL659KIY4X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM