Critical enzymes for biosynthesis of cucurbitacin derivatives in watermelon and their biological significance.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
14 08 2020
14 08 2020
Historique:
received:
29
01
2020
accepted:
22
07
2020
entrez:
16
8
2020
pubmed:
17
8
2020
medline:
17
6
2021
Statut:
epublish
Résumé
Various cucurbitacins have been isolated, and their structures have been elucidated. Owing to their economic potential and importance as active pharmacological compounds, their cytotoxicity in various cancer cells has been assessed. Here, we mined several candidate genes with potential involvement in cucurbitacin biosynthesis in watermelon (Citrullus lanatus) and performed in vitro enzymatic assays and instrumental analyses using various substrates to identify cucurbitacin functions and products. Enzymatic activities of two acetyltransferases (ACTs) and one UDP-glucosyltransferase (UGT) against cucurbitacins were confirmed, resulting in the synthesis of novel cucurbitacins in vivo and/or in vitro to our knowledge. As ACTs and UGT are involved in the dynamic conversion of cucurbitacins by catalyzing acetylation and glucosylation at moieties in the cucurbitacins skeleton, these findings improve our knowledge on how these genes contribute to the diversity of cucurbitacins.
Identifiants
pubmed: 32796947
doi: 10.1038/s42003-020-01170-2
pii: 10.1038/s42003-020-01170-2
pmc: PMC7429850
doi:
Substances chimiques
Cucurbitacins
60137-06-6
Carbon
7440-44-0
Acetyltransferases
EC 2.3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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