Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA.
T2 ribonucleases
Ustilago maydis
Zea mays
effector proteins
extracellular RNA
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
02
05
2020
accepted:
18
08
2020
pubmed:
28
8
2020
medline:
7
8
2021
entrez:
27
8
2020
Statut:
ppublish
Résumé
Ustilago maydis genome codes for many secreted ribonucleases. The contribution of two among these belonging to the T2 family (Nuc1 and Nuc2) in the pathogen virulence, has been assessed in this study. The nuc1 and nuc2 deletion mutants showed not only reduced pathogenicity compared to the SG200 WT strain but also exhibited significant delay in the completion of the pathogenic lifecycle. Both the proteins were also tested for their nucleolytic activities towards RNA substrates from maize and yeast. This also yielded valuable insights into the ability of the ribonucleases to utilise extracellular RNA as a nutrient source. Our study therefore established a role of two T2 type secreted ribonucleases of a phytopathogen in the acquisition of nutrient for the first time. This study also provides evidence that maize apoplast contains RNA, which can be utilised as a substrate by both Nuc1 and Nuc2.
Substances chimiques
Antigens, Fungal
0
Fungal Proteins
0
Virulence Factors
0
RNA
63231-63-0
Endoribonucleases
EC 3.1.-
ribonuclease T(2)
EC 3.1.27.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13256Informations de copyright
© 2020 John Wiley & Sons Ltd.
Références
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