Modifications to central carbon metabolism in an engineered Streptomyces host to enhance secondary metabolite production.
Biosynthetic gene cluster
Heterologous expression
Metabolic engineering
Secondary metabolites
Journal
Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
24
04
2020
revised:
05
08
2020
accepted:
06
08
2020
pubmed:
9
9
2020
medline:
19
12
2020
entrez:
8
9
2020
Statut:
ppublish
Résumé
To improve the production of secondary metabolites by alternation of the carbon metabolic flux, two types of deletion mutants of the central metabolic pathway, the Embden-Meyerhof (EM) or pentose phosphate (PP) pathway, in the genetically engineered Streptomyces avermitilis were constructed. Double-deletion mutants of phosphofructokinase (ΔpfkA1ΔpfkA3) in the EM pathway carrying a gene cluster for chloramphenicol biosynthesis markedly increased chloramphenicol production synthesized through the shikimate pathway. Although the ΔpfkA1ΔpfkA3 double-deletion mutant grew more slowly, its specific productivity of chloramphenicol (per dry cell weight) was 2.0-fold higher than that of the engineered S. avermitilis strain. However, the productivity of chloramphenicol was lower by the double-deletion mutant of transaldolase in the PP pathway, which supplies the precursor of the shikimate pathway. A carbon-flux analysis of the EM and PP pathways using [1-
Identifiants
pubmed: 32896473
pii: S1389-1723(20)30305-4
doi: 10.1016/j.jbiosc.2020.08.006
pii:
doi:
Substances chimiques
Carbon
7440-44-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
563-570Informations de copyright
Copyright © 2020 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.