Genome-wide effects of the antimicrobial peptide apidaecin on translation termination in bacteria.
E. coli
PrAMPs
antibacterial peptides
antibiotics
biochemistry
chemical biology
rescue sysytems
ribosome
ribosome profiling
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
08 10 2020
08 10 2020
Historique:
received:
01
09
2020
accepted:
20
09
2020
entrez:
8
10
2020
pubmed:
9
10
2020
medline:
20
2
2021
Statut:
epublish
Résumé
Biochemical studies suggested that the antimicrobial peptide apidaecin (Api) inhibits protein synthesis by binding in the nascent peptide exit tunnel and trapping the release factor associated with a terminating ribosome. The mode of Api action in bacterial cells had remained unknown. Here genome-wide analysis reveals that in bacteria, Api arrests translating ribosomes at stop codons and causes pronounced queuing of the trailing ribosomes. By sequestering the available release factors, Api promotes pervasive stop codon bypass, leading to the expression of proteins with C-terminal extensions. Api-mediated translation arrest leads to the futile activation of the ribosome rescue systems. Understanding the unique mechanism of Api action in living cells may facilitate the development of new medicines and research tools for genome exploration.
Identifiants
pubmed: 33031031
doi: 10.7554/eLife.62655
pii: 62655
pmc: PMC7544508
doi:
pii:
Substances chimiques
Antimicrobial Cationic Peptides
0
Codon, Terminator
0
apidaecin
123997-21-7
Banques de données
GEO
['GSE150034']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM133416
Pays : United States
Organisme : NCCIH NIH HHS
ID : T32 AT007533
Pays : United States
Organisme : National Science Foundation
ID : MCB 1951405
Pays : International
Informations de copyright
© 2020, Mangano et al.
Déclaration de conflit d'intérêts
KM, TF, XS, DK, IC, ZI, YG, AM, NV No competing interests declared
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