Effect of by-products from the dairy industry as alternative inducers of recombinant β-galactosidase expression.
Cheese whey
Cheese whey permeate
Escherichia coli
Lactose
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
29
04
2020
accepted:
09
10
2020
pubmed:
15
10
2020
medline:
21
10
2021
entrez:
14
10
2020
Statut:
ppublish
Résumé
The aim of the present study was to evaluate the efficiency of lactose derived from cheese whey and cheese whey permeate as inducer of recombinant Kluyveromyces sp. β-galactosidase enzyme produced in Escherichia coli. Two E. coli strains, BL21(DE3) and Rosetta (DE3), were used in order to produce the recombinant enzyme. Samples were evaluated for enzyme activity, total protein content, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis after induction with isopropyl-β-D-1-thiogalactoside (IPTG) (0.05 and 1 mM) and lactose, cheese whey, and cheese whey permeate solutions (1, 10, and 20 g/L lactose) at shake-flask cultivation, and whey permeate solution (10 g/L lactose) at bioreactor scale. The highest specific activities obtained with IPTG as inducer (0.05 mM) after 9 h of induction, were 23 and 33 U/mg The induction with cheese whey permeate was more efficient for recombinant β-galactosidase expression than the other inducers tested, and thus, represents an alternative form to reduce costs in recombinant protein production.
Identifiants
pubmed: 33052483
doi: 10.1007/s10529-020-03028-3
pii: 10.1007/s10529-020-03028-3
doi:
Substances chimiques
Culture Media
0
Fungal Proteins
0
Recombinant Proteins
0
beta-Galactosidase
EC 3.2.1.23
Lactose
J2B2A4N98G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
589-599Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 311655/2017-3
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 315225/2018-1
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 420506/2016-0
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