Fungal oxylipins direct programmed developmental switches in filamentous fungi.
Aspergillus flavus
/ genetics
Aspergillus fumigatus
/ genetics
Fungal Proteins
/ genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Hyphae
/ growth & development
Linoleic Acids
/ metabolism
Magnaporthe
/ genetics
Mutation
Oxylipins
/ metabolism
RNA-Seq
Receptors, G-Protein-Coupled
/ genetics
Signal Transduction
/ genetics
Transcription Factors
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 10 2020
14 10 2020
Historique:
received:
24
01
2020
accepted:
08
09
2020
entrez:
15
10
2020
pubmed:
16
10
2020
medline:
3
11
2020
Statut:
epublish
Résumé
Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show that an endogenously produced and secreted fungal oxylipin, 5,8-diHODE, induces fungal cellular differentiation, including lateral branching in pathogenic Aspergillus fumigatus and Aspergillus flavus, and appressorium formation in the rice blast pathogen Magnaporthe grisea. The Aspergillus branching response is specific to a subset of oxylipins and is signaled through G-protein coupled receptors. RNA-Seq profiling shows differential expression of many transcription factors in response to 5,8-diHODE. Screening of null mutants of 33 of those transcription factors identifies three transcriptional regulators that appear to mediate the Aspergillus branching response; one of the mutants is locked in a hypo-branching phenotype, while the other two mutants display a hyper-branching phenotype. Our work reveals an endogenous signal that triggers crucial developmental processes in filamentous fungi, and opens new avenues for research on the morphogenesis of filamentous fungi.
Identifiants
pubmed: 33056992
doi: 10.1038/s41467-020-18999-0
pii: 10.1038/s41467-020-18999-0
pmc: PMC7557911
doi:
Substances chimiques
Fungal Proteins
0
Linoleic Acids
0
Oxylipins
0
Receptors, G-Protein-Coupled
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5158Subventions
Organisme : Wellcome Trust
ID : 208396/Z/17/Z
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM112739
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI065728
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIEHS NIH HHS
ID : T32 ES007015
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007133
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008349
Pays : United States
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