Candidatus Frankia nodulisporulans sp. nov., an Alnus glutinosa-infective Frankia species unable to grow in pure culture and able to sporulate in-planta.


Journal

Systematic and applied microbiology
ISSN: 1618-0984
Titre abrégé: Syst Appl Microbiol
Pays: Germany
ID NLM: 8306133

Informations de publication

Date de publication:
Nov 2020
Historique:
received: 08 06 2020
revised: 04 09 2020
accepted: 07 09 2020
pubmed: 16 10 2020
medline: 3 3 2021
entrez: 15 10 2020
Statut: ppublish

Résumé

We describe a new Frankia species, for three non-isolated strains obtained from Alnus glutinosa in France and Sweden, respectively. These strains can nodulate several Alnus species (A. glutinosa, A. incana, A. alnobetula), they form hyphae, vesicles and sporangia in the root nodule cortex but have resisted all attempts at isolation in pure culture. Their genomes have been sequenced, they are significantly smaller than those of other Alnus-infective species (5Mb instead of 7.5Mb) and are very closely related to one another (ANI of 100%). The name Candidatus Frankia nodulisporulans is proposed. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and draft genome sequences reported in this study for AgTrS, AgUmASt1 and AgUmASH1 are MT023539/LR778176/LR778180 and NZ_CADCWS000000000.1/CADDZU010000001/CADDZW010000001, respectively.

Identifiants

pubmed: 33059155
pii: S0723-2020(20)30089-8
doi: 10.1016/j.syapm.2020.126134
pii:
doi:

Substances chimiques

DNA, Bacterial 0
RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

126134

Informations de copyright

Copyright © 2020 Elsevier GmbH. All rights reserved.

Auteurs

Aude Herrera-Belaroussi (A)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France. Electronic address: aude.herrera-belaroussi@univ-lyon1.fr.

Philippe Normand (P)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Katharina Pawlowski (K)

Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.

Maria P Fernandez (MP)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Daniel Wibberg (D)

The Center for Biotechnology CeBiTec, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.

Jörn Kalinowski (J)

The Center for Biotechnology CeBiTec, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.

Andreas Brachmann (A)

Biocenter of the LMU Munich, Genetics Section, Grosshaderner Str. 2-4, D-82152 Planegg-Martinsried, Germany.

Fede Berckx (F)

Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.

Natuschka Lee (N)

Department of Ecology and Environmental Science, Umeå University, 90187 Umeå, Sweden.

Jochen Blom (J)

Bioinformatics & Systems Biology, Justus-Liebig-University, 35392 Giessen, Hesse, Germany.

Adrien C Pozzi (AC)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Pascale Fournier (P)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Lorine Bethencourt (L)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Audrey Dubost (A)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Danis Abrouk (D)

Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.

Anita Sellstedt (A)

Department of Plant Physiology, UPSC, Umeå University, 90187 Umeå, Sweden. Electronic address: anita.sellstedt@umu.se.

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Classifications MeSH