Visualization of lipids in cottonseeds by matrix-assisted laser desorption/ionization mass spectrometry imaging.

Chromatography, Liquid Cottonseed Oil Lasers Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry
Cottonseed LC-MS/MS Lipids MALDI-MSI Spatial distribution

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Jan 2021
Historique:
received: 19 06 2020
revised: 27 08 2020
accepted: 28 08 2020
entrez: 20 10 2020
pubmed: 21 10 2020
medline: 15 5 2021
Statut: ppublish

Résumé

The cotton plant is an essential crop cultivated globally for its fiber and seeds. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to study the spatial distribution patterns of lipids in cottonseeds. 448 lipid ions were identified by LC-MS/MS, and 24 of which were precisely visualized by using MALDI-MSI. The lipids, including phosphatidylcholines (PC), phosphatidylethanolamines (PE) and triacylglycerols (TG) showed heterogeneous distribution patterns within the cotyledonary and radicle tissues. Additionally, the roles these lipids played in the metabolic pathways were analyzed, and relationship of the spatial distribution of LPC (lysophosphatidylcholine) and corresponding PC was studied. The unique distribution patterns of these lipid metabolites revealed by MSI can provide new insights into areas relating to the spatial compartmentation of lipid metabolism in plants. We believe that the results of MSI, if combined with transcriptomics and proteomics, may offer significant help in genetic engineering work.

Identifiants

DOI: 10.1016/j.talanta.2020.121614 PMID: 33076144
pubmed: 33076144
pii: S0039-9140(20)30905-X
doi: 10.1016/j.talanta.2020.121614
pii:
doi:

Substances chimiques

Cottonseed Oil 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121614

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Bingbing Liu (B)

State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China.

Xuanlin Meng (X)

College of Plant Protection, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, 271000, China.

Kun Li (K)

Henan International Joint Laboratory of Crop Adversity Multi-omics, Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, 475001, China.

Jinggong Guo (J)

Henan International Joint Laboratory of Crop Adversity Multi-omics, Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, 475001, China.

Zongwei Cai (Z)

State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China. Electronic address: zwcai@hkbu.edu.hk.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Visualization of lipids in cottonseeds by...
questionsmedicales.fr › Aliments et boissons › Aliments › Matières grasses alimentaires › Matières grasses alimentaires insaturées › Huile de coton › Visualization of lipids in cottonseeds by...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures pour les applications des radiations › Lasers › Visualization of lipids in cottonseeds by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Visualization of lipids in cottonseeds by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Visualization of lipids in cottonseeds by...