AmpliconDesign - an interactive web server for the design of high-throughput targeted DNA methylation assays.

DNA methylation analysis pipeline bisulfite sequencing epityper massarray primer design targeted sequencing

Journal

Epigenetics
ISSN: 1559-2308
Titre abrégé: Epigenetics
Pays: United States
ID NLM: 101265293

Informations de publication

Date de publication:
09 2021
Historique:
pubmed: 27 10 2020
medline: 13 1 2022
entrez: 26 10 2020
Statut: ppublish

Résumé

Targeted analysis of DNA methylation patterns based on bisulfite-treated genomic DNA (BT-DNA) is considered as a gold-standard for epigenetic biomarker development. Existing software tools facilitate primer design, primer quality control or visualization of primer localization. However, high-throughput design of primers for BT-DNA amplification is hampered by limits in throughput and functionality of existing tools, requiring users to repeatedly perform specific tasks manually. Consequently, the design of PCR primers for BT-DNA remains a tedious and time-consuming process. To bridge this gap, we developed

Identifiants

pubmed: 33100132
doi: 10.1080/15592294.2020.1834921
pmc: PMC8451469
doi:

Substances chimiques

Sulfites 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

933-939

Références

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Auteurs

Maximilian Schönung (M)

Section Translational Cancer Epigenomics, Division of Translational Medical Oncology, German Cancer Research Center (DKFZ) & National Center for Tumor Diseases (NCT), Heidelberg, Germany.
Faculty of Biosciences, Heidelberg University, Heidelberg, Germany.

Jana Hess (J)

Saarland University, Saarbrücken, Germany.

Pascal Bawidamann (P)

Department of Informatics, Technical University of Munich, Garching, Germany.

Sina Stäble (S)

Section Translational Cancer Epigenomics, Division of Translational Medical Oncology, German Cancer Research Center (DKFZ) & National Center for Tumor Diseases (NCT), Heidelberg, Germany.
Division of Experimental Hematology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Joschka Hey (J)

Faculty of Biosciences, Heidelberg University, Heidelberg, Germany.
Division of Cancer Epigenomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.
German-Israeli Helmholtz Research School in Cancer Biology.

Jens Langstein (J)

Section Translational Cancer Epigenomics, Division of Translational Medical Oncology, German Cancer Research Center (DKFZ) & National Center for Tumor Diseases (NCT), Heidelberg, Germany.
Faculty of Biosciences, Heidelberg University, Heidelberg, Germany.

Yassen Assenov (Y)

Division of Cancer Epigenomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Dieter Weichenhan (D)

Division of Cancer Epigenomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Pavlo Lutsik (P)

Division of Cancer Epigenomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Daniel B Lipka (DB)

Section Translational Cancer Epigenomics, Division of Translational Medical Oncology, German Cancer Research Center (DKFZ) & National Center for Tumor Diseases (NCT), Heidelberg, Germany.

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Classifications MeSH