Auxin apical dominance governed by the OsAsp1-OsTIF1 complex determines distinctive rice caryopses development on different branches.
Aspartic Acid Proteases
/ genetics
Edible Grain
/ genetics
Endoplasmic Reticulum
/ genetics
Gene Expression Regulation, Plant
/ genetics
Indoleacetic Acids
/ metabolism
Nuclear Proteins
/ genetics
Oryza
/ genetics
Plant Development
/ genetics
Plant Growth Regulators
/ genetics
Plant Proteins
/ genetics
Pollination
/ genetics
Transcription Factors
/ genetics
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
04
02
2020
accepted:
26
09
2020
revised:
06
11
2020
pubmed:
28
10
2020
medline:
1
1
2021
entrez:
27
10
2020
Statut:
epublish
Résumé
In rice (Oryza sativa), caryopses located on proximal secondary branches (CSBs) have smaller grain size and poorer grain filling than those located on apical primary branches (CPBs), greatly limiting grain yield. However, the molecular mechanism responsible for developmental differences between CPBs and CSBs remains elusive. In this transcriptome-wide expression study, we identified the gene Aspartic Protease 1 (OsAsp1), which reaches an earlier and higher transcriptional peak in CPBs than in CSBs after pollination. Disruption of OsAsp1 expression in the heterozygous T-DNA line asp1-1+/-eliminated developmental differences between CPBs and CSBs. OsAsp1 negatively regulated the transcriptional inhibitor of auxin biosynthesis, OsTAA1 transcriptional inhibition factor 1 (OsTIF1), to preserve indole-3-acetic acid (IAA) apical dominance in CPBs and CSBs. IAA also facilitated OsTIF1 translocation from the endoplasmic reticulum (ER) to the nucleus by releasing the interaction of OsTIF1 with OsAsp1 to regulate caryopses IAA levels via a feedback loop. IAA promoted transcription of OsAsp1 through MADS29 to maintain an OsAsp1 differential between CPBs and CSBs during pollination. Together, these findings provide a mechanistic explanation for the distributed auxin differential between CPBs and CSBs to regulate distinct caryopses development in different rice branches and potential targets for engineering yield improvement in crops.
Identifiants
pubmed: 33108367
doi: 10.1371/journal.pgen.1009157
pii: PGENETICS-D-20-00169
pmc: PMC7647119
doi:
Substances chimiques
Indoleacetic Acids
0
Nuclear Proteins
0
Plant Growth Regulators
0
Plant Proteins
0
Transcription Factors
0
transcriptional intermediary factor 1
0
indoleacetic acid
6U1S09C61L
Aspartic Acid Proteases
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009157Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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