Make it or break it: Plant AEPs on stage in biotechnology.
Asparaginyl endopeptidase
Cyclotide
Enzyme
Ligase
Peptide
Transpeptidation
Journal
Biotechnology advances
ISSN: 1873-1899
Titre abrégé: Biotechnol Adv
Pays: England
ID NLM: 8403708
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
13
07
2020
revised:
02
10
2020
accepted:
20
10
2020
pubmed:
4
11
2020
medline:
2
2
2021
entrez:
3
11
2020
Statut:
ppublish
Résumé
Asparaginyl endopeptidases (AEPs) are cysteine proteases that control a myriad of cellular functions in plants, including maturation of seed storage proteins and programmed cell death. Recently, several noteworthy AEPs have been discovered that primarily function as transpeptidases rather than hydrolases, to instead catalyse the formation of new peptide bonds. These AEPs appear to have evolved for the cyclisation of a large class of plant defence peptides called cyclotides. Here we describe recent insights into the structural differences between AEPs that preference peptide ligation over hydrolysis. This knowledge is instrumental for the deployment of AEP ligases as biotechnological tools for in vitro applications such as protein labelling and or cyclization, and for plant molecular farming applications.
Identifiants
pubmed: 33141031
pii: S0734-9750(20)30153-1
doi: 10.1016/j.biotechadv.2020.107651
pii:
doi:
Substances chimiques
Plant Proteins
0
Ligases
EC 6.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
107651Informations de copyright
Copyright © 2020. Published by Elsevier Inc.