Comparative genomics of Fusarium oxysporum f. sp. melonis strains reveals nine lineages and a new sequence type of AvrFom2.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
revised:
28
11
2020
received:
08
05
2020
accepted:
29
11
2020
pubmed:
2
12
2020
medline:
16
10
2021
entrez:
1
12
2020
Statut:
ppublish
Résumé
Fusarium oxysporum f. sp. melonis (Fom) is one of the most important pathogens of melon worldwide. In this study, we investigated the genomic diversity of Fom. One of the aims was to find clues for the origin(s) and dispersal of clonal lineages and races of Fom. We therefore included a large number of Fom strains from Iran, where melon has been cultivated for at least 5000 years. In 33 new genome sequences of Fom strains from different geographical regions of Iran and across the world, 40 new candidate effector genes were identified. Presence/absence of candidate effector genes and phylogenetic analyses resolved nine Fom lineages. The presence of a highly similar set of effector genes in some distant lineages is suggestive of horizontal chromosome transfer, a process known to occur in the Fusarium oxysporum species complex. Race 1.2, which breaks both Fom1 and Fom2 resistance genes, occurs in three of the nine lineages, two of which are predominant in Iran. We also identified a new sequence type of the AVRFom2 avirulence gene in one lineage. Expression of this sequence type during melon infection and genetic complementation suggest that this sequence type is not recognized by the Fom2 resistance protein.
Identifiants
pubmed: 33258545
doi: 10.1111/1462-2920.15339
pmc: PMC8246983
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2035-2053Informations de copyright
© 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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