Identification of two subtilisin-like serine proteases engaged in the degradation of recombinant proteins in Nicotiana benthamiana.
Agrobacterium tumefaciens
/ genetics
Amino Acid Chloromethyl Ketones
/ pharmacology
Antibodies, Monoclonal
/ biosynthesis
Gene Expression
HIV Antibodies
/ biosynthesis
Isoenzymes
/ antagonists & inhibitors
Phenylmethylsulfonyl Fluoride
/ pharmacology
Plant Proteins
/ antagonists & inhibitors
Plants, Genetically Modified
Protease Inhibitors
/ pharmacology
Proteolysis
Recombinant Proteins
/ biosynthesis
Subtilisins
/ antagonists & inhibitors
Nicotiana
/ drug effects
alpha 1-Antitrypsin
/ biosynthesis
Nicotiana benthamiana
biopharmaceutical
molecular farming
monoclonal antibody
recombinant protein expression
serine protease
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
09
10
2020
revised:
13
11
2020
accepted:
18
11
2020
pubmed:
3
12
2020
medline:
28
7
2021
entrez:
2
12
2020
Statut:
ppublish
Résumé
The tobacco variant Nicotiana benthamiana has recently emerged as a versatile host for the manufacturing of protein therapeutics, but the fidelity of many recombinant proteins generated in this system is compromised by inadvertent proteolysis. Previous studies have revealed that the anti-HIV-1 antibodies 2F5 and PG9 as well as the protease inhibitor α
Identifiants
pubmed: 33263189
doi: 10.1002/1873-3468.14014
pmc: PMC8221030
doi:
Substances chimiques
Amino Acid Chloromethyl Ketones
0
Antibodies, Monoclonal
0
HIV Antibodies
0
Isoenzymes
0
N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone
0
Plant Proteins
0
Protease Inhibitors
0
Recombinant Proteins
0
alpha 1-Antitrypsin
0
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
0
Phenylmethylsulfonyl Fluoride
57KD15003I
Subtilisins
EC 3.4.21.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
379-388Subventions
Organisme : Austrian Science Fund FWF
ID : W 1224
Pays : Austria
Informations de copyright
© 2020 Federation of European Biochemical Societies.
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