Expression analysis of microbial rhodopsin-like genes in Guillardia theta.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
08
2020
accepted:
19
11
2020
entrez:
3
12
2020
pubmed:
4
12
2020
medline:
26
1
2021
Statut:
epublish
Résumé
The Cryptomonad Guillardia theta has 42 genes encoding microbial rhodopsin-like proteins in their genomes. Light-driven ion-pump activity has been reported for some rhodopsins based on heterologous E. coli or mammalian cell expression systems. However, neither their physiological roles nor the expression of those genes in native cells are known. To reveal their physiological roles, we investigated the expression patterns of these genes under various growth conditions. Nitrogen (N) deficiency induced color change in exponentially growing G. theta cells from brown to green. The 29 rhodopsin-like genes were expressed in native cells. We found that the expression of 6 genes was induced under N depletion, while that of another 6 genes was reduced under N depletion.
Identifiants
pubmed: 33270796
doi: 10.1371/journal.pone.0243387
pii: PONE-D-20-24671
pmc: PMC7714340
doi:
Substances chimiques
Rhodopsins, Microbial
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0243387Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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