Alginate Degradation: Insights Obtained through Characterization of a Thermophilic Exolytic Alginate Lyase.
DEH
DHF
NMR
Saccharina latissima
alginate lyase
biorefining
exo-activity
nuclear magnetic resonance
thermal stability
Journal
Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801
Informations de publication
Date de publication:
26 02 2021
26 02 2021
Historique:
received:
30
09
2020
accepted:
19
12
2020
pubmed:
6
1
2021
medline:
7
4
2021
entrez:
5
1
2021
Statut:
epublish
Résumé
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. Herein, we describe a thermostable alginate lyase that belongs to polysaccharide lyase family 17 (PL17) and was derived from an Arctic Mid-Ocean Ridge (AMOR) metagenomics data set. This enzyme, AMOR_PL17A, is a thermostable exolytic oligoalginate lyase (EC 4.2.2.26), which can degrade alginate, poly-β-d-mannuronate, and poly-α-l-guluronate within a broad range of pHs, temperatures, and salinity conditions. Site-directed mutagenesis showed that tyrosine Y251, previously suggested to act as a catalytic acid, indeed is essential for catalysis, whereas mutation of tyrosine Y446, previously proposed to act as a catalytic base, did not affect enzyme activity. The observed reaction products are protonated and deprotonated forms of the 4,5-unsaturated uronic acid monomer, Δ, two hydrates of DEH (4-deoxy-l-
Identifiants
pubmed: 33397696
pii: AEM.02399-20
doi: 10.1128/AEM.02399-20
pmc: PMC8105002
pii:
doi:
Substances chimiques
Alginates
0
DNA, Plant
0
Plant Proteins
0
Polysaccharide-Lyases
EC 4.2.2.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 American Society for Microbiology.
Références
J Agric Food Chem. 2019 Mar 13;67(10):2936-2945
pubmed: 30781951
Front Microbiol. 2018 Feb 08;9:167
pubmed: 29472911
J Am Chem Soc. 2011 Dec 7;133(48):19334-7
pubmed: 22047074
Biochemistry. 1992 Mar 3;31(8):2239-53
pubmed: 1540579
J Agric Food Chem. 2018 Aug 8;66(31):8328-8335
pubmed: 30004220
Biotechnol Biofuels. 2012 Jul 02;5(1):45
pubmed: 22747961
Res Microbiol. 2006 Sep;157(7):642-9
pubmed: 16545947
Carbohydr Res. 2006 Aug 14;341(11):1809-19
pubmed: 16697989
J Biochem. 2016 Jan;159(1):77-86
pubmed: 26232404
Nucleic Acids Res. 2012 Jul;40(Web Server issue):W445-51
pubmed: 22645317
Appl Environ Microbiol. 2018 Jan 17;84(3):
pubmed: 29150496
Eur J Biochem. 1994 Dec 1;226(2):277-83
pubmed: 8001545
Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5
pubmed: 24270786
Biochem J. 2010 Dec 15;432(3):437-44
pubmed: 20925655
Mar Biotechnol (NY). 2012 Apr;14(2):189-202
pubmed: 21826589
Bioresour Technol. 2011 Jan;102(1):226-34
pubmed: 20685112
Front Microbiol. 2020 Feb 21;11:249
pubmed: 32153535
Biomacromolecules. 2012 Jan 9;13(1):106-16
pubmed: 22148348
Glycobiology. 2010 Dec;20(12):1547-73
pubmed: 20805221
J Biomol NMR. 2003 Mar;25(3):173-95
pubmed: 12652131
Protein Expr Purif. 2003 May;29(1):33-41
pubmed: 12729723
Nature. 2014 Jan 9;505(7482):239-43
pubmed: 24291791
J Bacteriol. 2003 Jun;185(12):3515-23
pubmed: 12775688
Nat Methods. 2011 Sep 29;8(10):785-6
pubmed: 21959131
Curr Opin Biotechnol. 2002 Aug;13(4):333-7
pubmed: 12323355
Appl Environ Microbiol. 2014 Jul;80(14):4207-14
pubmed: 24795372
J Biosci Bioeng. 2018 Sep;126(3):310-316
pubmed: 29680368
Appl Microbiol Biotechnol. 2012 Mar;93(5):2233-9
pubmed: 22281843
J Biol Chem. 2014 Mar 21;289(12):8645-55
pubmed: 24478312
Front Microbiol. 2015 May 27;6:523
pubmed: 26074905
PLoS One. 2019 Sep 6;14(9):e0222216
pubmed: 31491027
Bioresour Technol. 2015 May;184:415-420
pubmed: 25318906
Carbohydr Res. 1996 Aug 19;289:11-23
pubmed: 8805773
Glycobiology. 2016 Jan;26(1):3-12
pubmed: 26347522
Science. 2012 Jan 20;335(6066):308-13
pubmed: 22267807
J Agric Food Chem. 2018 Feb 14;66(6):1435-1443
pubmed: 29363310
Biotechnol Lett. 2015 Mar;37(3):665-71
pubmed: 25335746
Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1207-16
pubmed: 22948922
J Biol Chem. 2010 Aug 6;285(32):24519-28
pubmed: 20507980
J Biol Chem. 1962 Feb;237:309-16
pubmed: 14488584
J Bacteriol. 1999 Jul;181(13):3912-9
pubmed: 10383957
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
Appl Environ Microbiol. 2019 Mar 6;85(6):
pubmed: 30635385
J Biol Chem. 2013 Aug 9;288(32):23021-37
pubmed: 23782694
J Biotechnol. 2004 Sep 30;113(1-3):105-20
pubmed: 15380651
BMC Bioinformatics. 2010 Mar 08;11:119
pubmed: 20211023
Annu Rev Microbiol. 2000;54:289-340
pubmed: 11018131
Proc Natl Acad Sci U S A. 2016 May 31;113(22):6188-93
pubmed: 27185956
Bioresour Technol. 2016 Aug;213:155-161
pubmed: 26961713
Bioengineered. 2015;6(3):125-31
pubmed: 25831216