Transcriptional analysis of the lichenase-like gene cel12A of the filamentous fungus Stachybotrys atra BP-A and its relevance for lignocellulose depolymerization.
Catabolite Repression
Cellulose
/ metabolism
Fungal Proteins
/ chemistry
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Glucose
/ metabolism
Glycoside Hydrolases
/ chemistry
Hydrogen-Ion Concentration
Lignin
/ chemistry
Multigene Family
Polymerization
Stachybotrys
/ chemistry
Transcription, Genetic
Agricultural waste
Biomass depolymerization
Lichenase
RT-qPCR
Stachybotrys
Transcription regulation
Journal
International microbiology : the official journal of the Spanish Society for Microbiology
ISSN: 1618-1905
Titre abrégé: Int Microbiol
Pays: Switzerland
ID NLM: 9816585
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
31
07
2020
accepted:
14
12
2020
revised:
03
11
2020
pubmed:
7
1
2021
medline:
27
8
2021
entrez:
6
1
2021
Statut:
ppublish
Résumé
To rationally optimize the production of industrial enzymes by molecular means requires previous knowledge of the regulatory circuits controlling the expression of the corresponding genes. The genus Stachybotrys is an outstanding producer of cellulose-degrading enzymes. Previous studies isolated and characterized the lichenase-like/non-typical cellulase Cel12A of S. atra (AKA S. chartarum) belonging to glycosyl hydrolase family 12 (GH12). In this study, we used RT-qPCR to determine the pattern of expression of cel12A under different carbon sources and initial ambient pH. Among the carbon sources examined, rice straw triggered a greater increase in the expression of cel12A than 1% lactose or 0.1% glucose, indicating specific induction by rice straw. In contrast, cel12A was repressed in the presence of glucose even when combined with this inducer. The proximity of 2 adjacent 5'-CTGGGGTCTGGGG-3' CreA consensus target sites to the translational start site of cel12A strongly suggests that the carbon catabolite repression observed is directly mediated by CreA. Ambient pH did not have a significant effect on cel12A expression. These findings present new knowledge on transcriptional regulatory networks in Stachybotrys associated with cellulose/hemicellulose depolymerization. Rational engineering of CreA to remove CCR could constitute a novel strategy for improving the production of Cel12A.
Identifiants
pubmed: 33404932
doi: 10.1007/s10123-020-00155-9
pii: 10.1007/s10123-020-00155-9
doi:
Substances chimiques
Fungal Proteins
0
lignocellulose
11132-73-3
Cellulose
9004-34-6
Lignin
9005-53-2
Glycoside Hydrolases
EC 3.2.1.-
licheninase
EC 3.2.1.73
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
197-205Subventions
Organisme : Generalitat Valenciana
ID : PROMETEO/2018/066
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