Resonance assignments and secondary structure of thermophile single-stranded DNA binding protein from Sulfolobus solfataricus at 323K.
High Temperatures NMR spectroscopy
OB-fold
Single‐stranded binding
Thermophile
Journal
Biomolecular NMR assignments
ISSN: 1874-270X
Titre abrégé: Biomol NMR Assign
Pays: Netherlands
ID NLM: 101472371
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
01
11
2020
accepted:
22
12
2020
pubmed:
7
1
2021
medline:
17
12
2021
entrez:
6
1
2021
Statut:
ppublish
Résumé
Single-stranded DNA (ssDNA)-binding proteins (SSBs) are essential for DNA replication, recombination, and repair processes in all organisms. Sulfolobus solfataricus (S. solfataricus), a hyperthermophilic species, overexpresses its SSB (S. solfataricus SSB (SsoSSB)) to protect ssDNA during DNA metabolisms. Even though the crystal structure of apo SsoSSB and its ssDNA-bound solution structure have been reported at room temperature, structural information at high temperature is not yet available. To find out how SsoSSB maintains its structure and ssDNA binding affinity at high temperatures, we performed multidimensional NMR experiments for SsoSSB at 323K. In this study, we present the backbone and side chain chemical shifts and predict the secondary structure of SsoSSB from the chemical shifts. We found that SsoSSB is ordered, even at high temperatures, and has the same fold at high temperature as at room temperature. Our data will help improve structural analyses and our understanding of the features of thermophilic proteins.
Identifiants
pubmed: 33405014
doi: 10.1007/s12104-020-09999-8
pii: 10.1007/s12104-020-09999-8
doi:
Substances chimiques
Archaeal Proteins
0
DNA, Single-Stranded
0
DNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
159-164Subventions
Organisme : National Research Foundation of Korea
ID : 2018R1A2B6004388
Organisme : Gwangju Institute of Science and Technology
ID : N/A
Organisme : National Science Foundation (US)
ID : DBI-2051595
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