Tissue-Resident Memory CD8+ T Cells From Skin Differentiate Psoriatic Arthritis From Psoriasis.
Adult
Aminopeptidases
/ genetics
Antigens, CD
/ genetics
Antigens, Differentiation, T-Lymphocyte
/ immunology
Arthritis, Psoriatic
/ genetics
CD8-Positive T-Lymphocytes
/ immunology
Case-Control Studies
Female
Forkhead Transcription Factors
/ genetics
GATA3 Transcription Factor
/ genetics
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Humans
Immunologic Memory
/ immunology
Immunophenotyping
Integrin alpha Chains
/ genetics
Interleukin-17
/ immunology
Interleukins
/ immunology
Male
Middle Aged
Minor Histocompatibility Antigens
/ genetics
Nuclear Receptor Subfamily 1, Group F, Member 3
/ genetics
Oligosaccharides
/ metabolism
Psoriasis
/ genetics
Receptor, Interferon alpha-beta
/ genetics
Receptors, CCR10
/ metabolism
Receptors, CCR4
/ metabolism
Sialyl Lewis X Antigen
/ analogs & derivatives
Skin
/ immunology
Spondylarthropathies
/ genetics
Synovial Fluid
/ cytology
T-Lymphocyte Subsets
/ immunology
T-Lymphocytes, Cytotoxic
/ immunology
T-Lymphocytes, Regulatory
/ immunology
Interleukin-22
Journal
Arthritis & rheumatology (Hoboken, N.J.)
ISSN: 2326-5205
Titre abrégé: Arthritis Rheumatol
Pays: United States
ID NLM: 101623795
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
20
08
2020
accepted:
07
01
2021
pubmed:
17
1
2021
medline:
10
8
2021
entrez:
16
1
2021
Statut:
ppublish
Résumé
To compare immune cell phenotype and function in psoriatic arthritis (PsA) versus psoriasis in order to better understand the pathogenesis of PsA. In-depth immunophenotyping of different T cell and dendritic cell subsets was performed in patients with PsA, psoriasis, or axial spondyloarthritis and healthy controls. Subsequently, we analyzed cells from peripheral blood, synovial fluid (SF), and skin biopsy specimens using flow cytometry, along with high-throughput transcriptome analyses and functional assays on the specific cell populations that appeared to differentiate PsA from psoriasis. Compared to healthy controls, the peripheral blood of patients with PsA was characterized by an increase in regulatory CD4+ T cells and interleukin-17A (IL-17A) and IL-22 coproducing CD8+ T cells. One population specifically differentiated PsA from psoriasis: i.e., CD8+CCR10+ T cells were enriched in PsA. CD8+CCR10+ T cells expressed high levels of DNAX accessory molecule 1 and were effector memory cells that coexpressed skin-homing receptors CCR4 and cutaneous lymphocyte antigen. CD8+CCR10+ T cells were detected under inflammatory and homeostatic conditions in skin, but were not enriched in SF. Gene profiling further revealed that CD8+CCR10+ T cells expressed GATA3, FOXP3, and core transcriptional signature of tissue-resident memory T cells, including CD103. Specific genes, including RORC, IFNAR1, and ERAP1, were up-regulated in PsA compared to psoriasis. CD8+CCR10+ T cells were endowed with a Tc2/22-like cytokine profile, lacked cytotoxic potential, and displayed overall regulatory function. Tissue-resident memory CD8+ T cells derived from the skin are enhanced in the circulation of patients with PsA compared to patients with psoriasis alone. This may indicate that aberrances in cutaneous tissue homeostasis contribute to arthritis development.
Identifiants
pubmed: 33452865
doi: 10.1002/art.41652
pmc: PMC8362143
doi:
Substances chimiques
6-sulfo sialyl Lewis X
0
Antigens, CD
0
Antigens, Differentiation, T-Lymphocyte
0
CCR10 protein, human
0
CCR4 protein, human
0
CD226 antigen
0
FOXP3 protein, human
0
Forkhead Transcription Factors
0
GATA3 Transcription Factor
0
GATA3 protein, human
0
IFNAR1 protein, human
0
IL17A protein, human
0
Integrin alpha Chains
0
Interleukin-17
0
Interleukins
0
Minor Histocompatibility Antigens
0
Nuclear Receptor Subfamily 1, Group F, Member 3
0
Oligosaccharides
0
RORC protein, human
0
Receptors, CCR10
0
Receptors, CCR4
0
Sialyl Lewis X Antigen
0
alpha E integrins
0
Receptor, Interferon alpha-beta
156986-95-7
Aminopeptidases
EC 3.4.11.-
ERAP1 protein, human
EC 3.4.11.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1220-1232Informations de copyright
© 2021 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology.
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Nucleic Acids Res. 2015 Jan;43(Database issue):D662-9
pubmed: 25352552
Clin Exp Rheumatol. 2018 May-Jun;36(3):486-489
pubmed: 29148410
Acta Derm Venereol. 2016 May;96(4):436-41
pubmed: 26928459
Dermatologica. 1978;157(4):238-44
pubmed: 357213
Br J Rheumatol. 1997 Jul;36(7):748-57
pubmed: 9255108
Arthritis Rheumatol. 2020 Mar;72(3):435-447
pubmed: 31677365
Arthritis Rheum. 2008 Jun;58(6):1796-802
pubmed: 18512820
Arthritis Rheum. 1996 Jan;39(1):137-45
pubmed: 8546722
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Nat Rev Rheumatol. 2015 Dec;11(12):731-40
pubmed: 26526644
Nature. 2012 Apr 26;484(7395):514-8
pubmed: 22466287
Autoimmun Rev. 2017 Jan;16(1):10-15
pubmed: 27666819
Sci Transl Med. 2015 Mar 18;7(279):279ra39
pubmed: 25787765
Sci Immunol. 2019 Jul 5;4(37):
pubmed: 31278120
Immunity. 2017 Feb 21;46(2):287-300
pubmed: 28214226
Nat Med. 2012 Jul 01;18(7):1069-76
pubmed: 22772566
Genome Biol. 2014;15(12):550
pubmed: 25516281
Curr Protoc Bioinformatics. 2016 Sep 07;55:14.10.1-14.10.91
pubmed: 27603023
Blood. 2011 Sep 29;118(13):3538-48
pubmed: 21828127
J Allergy Clin Immunol. 2021 Feb;147(2):759-763.e9
pubmed: 32533971
Arthritis Rheumatol. 2014 May;66(5):1272-81
pubmed: 24470327
Br J Dermatol. 2019 Aug;181(2):410-412
pubmed: 30737771
Inflamm Res. 2020 Mar;69(3):245-254
pubmed: 31989191
Arthritis Res Ther. 2013 Sep 26;15(5):R136
pubmed: 24286492
Front Immunol. 2018 Oct 31;9:2519
pubmed: 30429855
Arthritis Rheumatol. 2016 Dec;68(12):2911-2916
pubmed: 27389865
Ann Rheum Dis. 2009 Jun;68(6):777-83
pubmed: 19297344
J Immunol. 2006 Jul 1;177(1):593-603
pubmed: 16785557
Nat Immunol. 2009 Aug;10(8):864-71
pubmed: 19578368
J Exp Med. 2020 Oct 5;217(10):
pubmed: 32728699
J Allergy Clin Immunol. 2019 Apr;143(4):1444-1454
pubmed: 30268387
Arthritis Rheum. 2006 Aug;54(8):2665-73
pubmed: 16871531
J Invest Dermatol. 2015 Feb;135(2):620-623
pubmed: 25243786
Nat Med. 2002 Feb;8(2):157-65
pubmed: 11821900
J Allergy Clin Immunol. 2014 Sep;134(3):634-644.e10
pubmed: 24767879
N Engl J Med. 2017 Mar 9;376(10):957-970
pubmed: 28273019
Nat Commun. 2019 Jan 2;10(1):9
pubmed: 30602780
Front Immunol. 2016 Nov 30;7:549
pubmed: 27965674
J Immunol. 2007 Apr 1;178(7):4112-9
pubmed: 17371966
Front Immunol. 2018 Apr 25;9:885
pubmed: 29922283
Rheumatology (Oxford). 2019 Jun 1;58(6):963-968
pubmed: 30204909
Nat Commun. 2015 Feb 05;6:6046
pubmed: 25651891
Nat Immunol. 2013 Dec;14(12):1294-301
pubmed: 24162776
J Immunol. 2016 Jun 15;196(12):4859-64
pubmed: 27183612
J Immunol. 2018 Mar 1;200(5):1639-1650
pubmed: 29427415
J Immunol. 2014 Apr 1;192(7):3111-20
pubmed: 24610014
Nat Immunol. 2019 Aug;20(8):992-1003
pubmed: 31263279
Nat Immunol. 2007 Mar;8(3):285-93
pubmed: 17259988
J Immunol. 2019 Nov 15;203(10):2561-2569
pubmed: 31685700
Lancet. 2018 Jun 2;391(10136):2273-2284
pubmed: 29893226