CRISPR technologies and the search for the PAM-free nuclease.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
22 01 2021
22 01 2021
Historique:
received:
15
09
2020
accepted:
03
12
2020
entrez:
23
1
2021
pubmed:
24
1
2021
medline:
9
2
2021
Statut:
epublish
Résumé
The ever-expanding set of CRISPR technologies and their programmable RNA-guided nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with an ever-present constraint: the requirement for a protospacer adjacent motif (PAM) flanking each target. While PAMs play an essential role in self/nonself discrimination by CRISPR-Cas immune systems, this constraint has launched a far-reaching expedition for nucleases with relaxed PAM requirements. Here, we review ongoing efforts toward realizing PAM-free nucleases through natural ortholog mining and protein engineering. We also address potential consequences of fully eliminating PAM recognition and instead propose an alternative nuclease repertoire covering all possible PAM sequences.
Identifiants
pubmed: 33483498
doi: 10.1038/s41467-020-20633-y
pii: 10.1038/s41467-020-20633-y
pmc: PMC7822910
doi:
Substances chimiques
RNA, Guide
0
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
555Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM119561
Pays : United States
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