Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice.

Biodegradation, Environmental Food Contamination / analysis Fruit and Vegetable Juices / analysis Malus / chemistry Molecular Docking Simulation Patulin / isolation & purification Saccharomycetales / enzymology Short Chain Dehydrogenase-Reductases / metabolism
Apple juice Degradation Patulin Short-chain dehydrogenase/reductase

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
30 Jun 2021
Historique:
received: 16 09 2020
revised: 24 12 2020
accepted: 05 01 2021
pubmed: 29 1 2021
medline: 27 3 2021
entrez: 28 1 2021
Statut: ppublish

Résumé

Biodegradation based on microbial enzymes is considered to be one of the promising ways for controlling patulin contamination. However, few patulin degrading enzymes have been isolated and characterized until now. Here, a short-chain dehydrogenase/reductase (SDR) gene, CgSDR, was cloned from a yeast strain Candida guilliermondii, and expressed in Escherichia coli. The expression of CgSDR conferred a strong patulin tolerance and degradation ability to E. coli, and purified CgSDR could transform patulin into E-ascladiol in vitro with NADPH as a coenzyme. Moreover, addition of CgSDR at 150 μg/mL could reduce 80% of patulin in apple juice and the biodegradation process did not affect the quality of the apple juice. A molecular docking analysis and site-directed mutagenesis indicated that CgSDR might interact with patulin via VAL188 as an active binding sites. The findings provide new insights for developing enzymic formulations for mycotoxin detoxification in fruit derived products.

Identifiants

DOI: 10.1016/j.foodchem.2021.129046 PMID: 33508606
pubmed: 33508606
pii: S0308-8146(21)00048-0
doi: 10.1016/j.foodchem.2021.129046
pii:
doi:

Substances chimiques

Patulin 95X2BV4W8R
Short Chain Dehydrogenase-Reductases EC 1.1.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

129046

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Mengyang Xing (M)

Key Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Yong Chen (Y)

Key Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100093, China.

Boqiang Li (B)

Key Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: bqli@ibcas.ac.cn.

Shiping Tian (S)

Key Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: tsp@ibcas.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Santé publique › Pollution de l'environnement › Assainissement et restauration de l'environnement › Dépollution biologique de l'environnement › Characterization of a short-chain...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › Characterization of a short-chain...
questionsmedicales.fr › Aliments et boissons › Boissons › Jus de fruits et de légumes › Characterization of a short-chain...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Rosanae › Rosales › Rosaceae › Malus › Characterization of a short-chain...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Characterization of a short-chain...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Mycotoxines › Patuline › Characterization of a short-chain...
questionsmedicales.fr › Eucaryotes › Champignons › Ascomycota › Saccharomycetales › Characterization of a short-chain...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › NAD (+) and NADP (+) dependent Alcohol oxidoreductases › Short chain dehydrogenase-reductases › Characterization of a short-chain...