Repression of a large number of genes requires interplay between homologous recombination and HIRA.
Cell Cycle Proteins
/ antagonists & inhibitors
Centromere
Gene Expression Regulation, Fungal
Gene Silencing
Histone Code
Homologous Recombination
Nucleosomes
/ metabolism
Repressor Proteins
/ physiology
Schizosaccharomyces
/ genetics
Schizosaccharomyces pombe Proteins
/ antagonists & inhibitors
Transcription Factors
/ antagonists & inhibitors
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
26 02 2021
26 02 2021
Historique:
accepted:
09
01
2021
revised:
06
01
2021
received:
20
01
2020
pubmed:
30
1
2021
medline:
10
3
2021
entrez:
29
1
2021
Statut:
ppublish
Résumé
During homologous recombination, Dbl2 protein is required for localisation of Fbh1, an F-box helicase that efficiently dismantles Rad51-DNA filaments. RNA-seq analysis of dbl2Δ transcriptome showed that the dbl2 deletion results in upregulation of more than 500 loci in Schizosaccharomyces pombe. Compared with the loci with no change in expression, the misregulated loci in dbl2Δ are closer to long terminal and long tandem repeats. Furthermore, the misregulated loci overlap with antisense transcripts, retrotransposons, meiotic genes and genes located in subtelomeric regions. A comparison of the expression profiles revealed that Dbl2 represses the same type of genes as the HIRA histone chaperone complex. Although dbl2 deletion does not alleviate centromeric or telomeric silencing, it suppresses the silencing defect at the outer centromere caused by deletion of hip1 and slm9 genes encoding subunits of the HIRA complex. Moreover, our analyses revealed that cells lacking dbl2 show a slight increase of nucleosomes at transcription start sites and increased levels of methylated histone H3 (H3K9me2) at centromeres, subtelomeres, rDNA regions and long terminal repeats. Finally, we show that other proteins involved in homologous recombination, such as Fbh1, Rad51, Mus81 and Rad54, participate in the same gene repression pathway.
Identifiants
pubmed: 33511417
pii: 6123375
doi: 10.1093/nar/gkab027
pmc: PMC7913671
doi:
Substances chimiques
Cell Cycle Proteins
0
Dbl2 protein, S pombe
0
Nucleosomes
0
Repressor Proteins
0
Schizosaccharomyces pombe Proteins
0
Slm9 protein, S pombe
0
Transcription Factors
0
hip1 protein, S pombe
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1914-1934Subventions
Organisme : Austrian Science Fund FWF
ID : P 30516
Pays : Austria
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
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