Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.
Cell Line, Tumor
CpG Islands
DNA Methylation
Epigenesis, Genetic
Epigenome
Epigenomics
Gene Expression Regulation, Neoplastic
High-Throughput Nucleotide Sequencing
Humans
Leukemia, Myeloid, Acute
/ genetics
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
RNA-Seq
Reproducibility of Results
Single-Cell Analysis
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
01 02 2021
01 02 2021
Historique:
received:
07
06
2019
accepted:
06
01
2021
entrez:
2
2
2021
pubmed:
3
2
2021
medline:
10
8
2021
Statut:
epublish
Résumé
Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.
Identifiants
pubmed: 33526904
doi: 10.1038/s42003-021-01661-w
pii: 10.1038/s42003-021-01661-w
pmc: PMC7851132
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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