DNA barcoding: A different perspective to introducing undergraduate students to DNA sequence analysis.
DNA barcoding
DNA sequence databases
DNA sequencing
Journal
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
ISSN: 1539-3429
Titre abrégé: Biochem Mol Biol Educ
Pays: United States
ID NLM: 100970605
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
09
11
2020
received:
11
12
2019
accepted:
31
12
2020
pubmed:
3
2
2021
medline:
28
9
2021
entrez:
2
2
2021
Statut:
ppublish
Résumé
Education in biochemistry teaching laboratories focus primarily on applying biochemical techniques to understanding human disease, biochemistry, and biotechnology. With anthropogenic climate change, there is a renewed interest in quantifying biodiversity, especially with the use of molecular-based approaches such as DNA barcoding. This 3-week laboratory exercise allowed undergraduate students to explore DNA sequencing, analysis, and DNA barcoding. Students extracted DNA from insect legs and amplified a 650 bp section of Cytochrome C oxidase I gene by PCR, and confirmed the success of their PCR by DNA gel electrophoresis. The PCR products were submitted for sequencing and students analyzed the sequences using FinchTV, Genbank, and the Barcode of Life Database. Based on the DNA sequences of their PCR products students were able to identify the species of insects. This lab exercise provides a different context to introducing students to analyzing DNA sequences and using DNA databases.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
416-421Informations de copyright
© 2021 International Union of Biochemistry and Molecular Biology.
Références
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