Oligonucleotides Containing 1-Aminomethyl or 1-Mercaptomethyl-2-deoxy-d-ribofuranoses: Synthesis, Purification, Characterization, and Conjugation with Fluorophores and Lipids.
Journal
Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319
Informations de publication
Date de publication:
17 02 2021
17 02 2021
Historique:
pubmed:
6
2
2021
medline:
18
8
2021
entrez:
5
2
2021
Statut:
ppublish
Résumé
Oligonucleotide conjugates are widely used as therapeutic drugs, gene analysis, and diagnostic tools. A critical step in the biologically relevant oligonucleotide conjugates is the design and synthesis of functional molecules that connect oligonucleotide with ligands. Here, we report the synthesis and application for oligonucleotide functionalization of novel tethers based on aminomethyl and mercaptomethyl sugar derivatives. Starting from a common cyano sugar precursor, three novel phosphoramidites have been prepared in the two α- and β-anomeric forms. The mercaptomethyl sugar was protected with the
Identifiants
pubmed: 33543930
doi: 10.1021/acs.bioconjchem.0c00717
pmc: PMC9122261
doi:
Substances chimiques
Fluorescent Dyes
0
Ligands
0
Lipids
0
Oligonucleotides
0
Organophosphorus Compounds
0
phosphoramidite
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
350-366Références
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